GCN5,a Yeast Transcriptional Coactivator, Induces Chromatin Reconfiguration ofHIS3Promoterin Vivo
Gcn5p, the nuclear histone acetyltransferase (HAT A), is a component of the multiprotein adaptor complex,ADA.Its role as a transcriptional coactivator is required for full induction of most of the genes regulated byGCN4.In this study we present experimental evidence demonstrating that, during gene a...
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| Published in: | Biochemical and biophysical research communications Vol. 242; no. 1; pp. 84 - 87 |
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| Main Authors: | , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
Elsevier Inc
06-01-1998
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| Online Access: | Get full text |
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| Summary: | Gcn5p, the nuclear histone acetyltransferase (HAT A), is a component of the multiprotein adaptor complex,ADA.Its role as a transcriptional coactivator is required for full induction of most of the genes regulated byGCN4.In this study we present experimental evidence demonstrating that, during gene activation, the nuclease sensitive region ofHIS3promoter, harbouring the poly (dA:dT) and theGCN4binding site, is invaded by nucleosomes in agcn5disrupted strain. These data demonstrate, for the first time, that Gcn5p affects directly the chromatin organization of a chromosomal gene during its transcriptional activation. |
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| ISSN: | 0006-291X 1090-2104 |
| DOI: | 10.1006/bbrc.1997.7918 |