Feasibility of the 99mTc-HYNIC-βAla-Bombesin(7–14) for detection of LNCaP prostate tumour in experimental model

• Published in: Journal of radioanalytical and nuclear chemistry Vol. 305; no. 2; pp. 379 - 386
• Main Authors: Fuscaldi, Leonardo Lima, de Barros, André Luís Branco, Santos, Caiubi Rodrigues de Paula, de Oliveira, Mônica Cristina, Fernandes, Simone Odília Antunes, Cardoso, Valbert Nascimento
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01-08-2015
• Subjects: Chemistry; Chemistry and Materials Science; Diagnostic Radiology; Hadrons; Heavy Ions; Inorganic Chemistry; Nuclear Chemistry; Nuclear Physics; Physical Chemistry; Radiopeptide; Molecular imaging; Ex vivo biodistribution; Bombesin; Technetium-99m; LNCaP prostate tumour
• ISSN: 0236-5731; 1588-2780
• DOI: 10.1007/s10967-015-4030-8

Previous work revealed that 25-day tumour focus is the optimal developmental stage to assess LNCaP prostate tumour by scintigraphic images using technetium-99m (
99m
Tc)-2-hydrazinonicotinamide (HYNIC)-beta-alanine (βAla)-Bombesin
(7–14)
in a murine model. However, more studies are essential to evaluate the feasibility of this radiopeptide as a radiotracer for LNCaP tumours. Thus, in vitro and in vivo assays were performed and showed specific affinity of
99m
Tc-HYNIC-βAla-Bombesin
(7–14)
for LNCaP cells. Scintigraphic images and biodistribution data performed in LNCaP tumour-bearing mice revealed radiotracer accumulation in tumour focus, with only 7.6 mm. Therefore,
99m
Tc-HYNIC-βAla-Bombesin
(7–14)
was able to identify LNCaP tumour, at an early developmental stage.