Development of probiotic gummy candy using the indigenous Lactobacillus plantarum Dad-13 strain; evaluation of its gastrointestinal resistance and shelflife prediction

• Published in: Food Research (Online) Vol. 5; no. 5; pp. 265 - 273
• Main Authors: Kamil, R.Z., Fadhila, F.H., Rachmasari, A.D., Murdiati, A., Juffrie, M., Rahayu, E.S.
• Format: Journal Article
• Language: English
• Published: 30-10-2021
• ISSN: 2550-2166; 2550-2166
• DOI: 10.26656/fr.2017.5(5).731

Nowadays, functional food especially probiotic products have gained more interest, and
gummy candy L. plantarum Dad-13 is an innovation. However, the efficacy of probiotics
relies on their viable cells and resistance in the gastrointestinal tract. Therefore, this
research aimed to evaluate the gastrointestinal resistance and probiotic cells' shelf life and
investigate gummy probiotics' characteristics. A market survey was carried to understand
the consumer's knowledge on the health benefit of probiotics and their product, also intend
to buy the product. Gastrointestinal simulation with pepsin and the pancreatic enzyme was
conducted to evaluate the gastrointestinal tract's probiotic resistance, probiotic shelf life
was evaluated with the Accelerated Shelf-life Test (ASLT). Proximate analysis was
carried out according to the Association of Official Analytical Chemists (AOAC), and
physical characteristics were analysed before and after 90 days of storage at 4oC. The
result showed that the supplementation of L. plantarum Dad-13 in the gummy probiotic
had an excellent survival during gastrointestinal system simulation with the predicted
shelf life was 75.17 days or 2.5 months at 4oC of storage. However, the moisture content
of gummy probiotics was more than 20%. Considering the survival of L. plantarum Dad13 during gastrointestinal simulation, it can be concluded that gummy probiotics can be
used as a carrier for L. plantarum Dad-13.