A facile electrochemical aptasensor for chloramphenicol detection based on synergistically photosensitization enhanced by SYBR Green I and MoS2

• Published in: Journal of colloid and interface science Vol. 672; pp. 236 - 243
• Main Authors: Feng, Hui, Luo, Meng, Zhu, Guonian, Mokeira, Kerage Dorothy, Yang, Yaoxin, Lv, Yongxin, Tan, Qing, Lei, Xiangwen, Zeng, Hang, Cheng, Hefa, Xu, Shuxia
• Format: Journal Article
• Language: English
• Published: Elsevier Inc 15-10-2024
• Subjects: Au nanoparticles; Chloramphenicol; Electrochemical aptasensor; MoS2; Photosensitization; SYBR Green I; Photosensitization; Au nanoparticles; Chloramphenicol; Electrochemical aptasensor; MoS2; SYBR Green I
• ISSN: 0021-9797; 1095-7103; 1095-7103
• DOI: 10.1016/j.jcis.2024.05.109

A photocatalytic electrochemical aptasensor for the detection of chloramphenicol（CAP）antibiotic residues in water by utilizing SYBR Green I（SG）and chemically exfoliated MoS2（ce-MoS2） as synergistically signal-amplification platforms.
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•A novel photocatalytic electrochemical aptasensor was developed for the detection of chloramphenicol (CAP) antibiotic residues in water.•Photosensitized SYBR Green I (SG) and chemically stripped MoS2(ce-MoS2) were intercalated into the groove of the resulting dsDNA and use as a synergistic signal-amplification platform.•Upon light irradiation, the photocatalytic reaction was activated, generating singlet oxygen that efficiently cleaved the dsDNA, leading to a significant increase in the current of [Fe(CN)6]3-/4-.•The aptasensor demonstrated good selectivity towards CAP in the presence of interfering antibiotics.•The aptasensor exhibited a linear relationship between CAP and logarithmic concentrations from 20 to 1000 nM, with a detection limit (3σ) of 3.391 nM.
This study reports the development of a photocatalytic electrochemical aptasensor for the purpose of detecting chloramphenicol (CAP) antibiotic residues in water by utilizing SYBR Green I (SG) and chemically exfoliated MoS2 (ce-MoS2) as synergistically signal-amplification platforms. The Au nanoparticles (AuNPs) were electrodeposited onto the surface of an indium tin oxide (ITO) electrode. After that, the thiolate-modified cDNA, also known as capture DNA, was combined with the aptamer. Subsequently, photosensitized SG molecules and ce-MoS2 nanomaterial were inserted into the groove of the resultant double-stranded DNA (dsDNA). The activation of the photocatalytic process upon exposure to light resulted in the generation of singlet oxygen. The singlet oxygen effectively split the dsDNA, resulting in significant enhancement in the current of [Fe(CN)6]3-/4-. When the CAP was present, both SG molecules and ce-MoS2 broke away from the dsDNA, which turned off the photosensitization response, leading to significant reduction in the current of [Fe(CN)6]3-/4-. Under the optimal conditions, the aptasensor exhibited a linear relationship between the current of [Fe(CN)6]3-/4- with logarithmic concentrations of CAP from 20 to 1000 nM, with a detection of limit (3σ) of 3.391 nM. The aptasensor also demonstrated good selectivity towards CAP in the presence of interfering antibiotics, such as tetracycline, streptomycin, levofloxacin, ciprofloxacin, and sulfadimethoxine. Additionally, the results obtained from the analysis of natural water samples using the proposed aptasensor were consistent with the findings acquired through the use of a liquid chromatograph-mass spectrometer. Therefore, with its simplicity and high selectivity, this aptasensor can potentially detect alternative antibiotics in environmental water samples by replacing the aptamers based on photosensitization.