Neuroprotective effect of Cistus laurifolius on hydrogen peroxide-induced neurodegeneration in differentiated SH-SY5Y cells

• Published in: Biological diversity and conservation Vol. 16; no. 3; p. 229
• Main Authors: Eciroglu, Hamiyet, Yildiz, Fatma, Yucel, Ersin
• Format: Journal Article
• Language: English; Turkish
• Published: Eskisehir Biological Diversity and Conservation 01-12-2023
• Subjects: Alzheimer's disease; Cell differentiation; Cell viability; Cistus laurifolius; Gene expression; Hydrogen peroxide; Movement disorders; Neurodegeneration; Neurodegenerative diseases; Neurological diseases; Neuroprotection; Neurotoxicity; Parkinson's disease; Polymerase chain reaction; Reverse transcription; Toxicity
• DOI: 10.46309/biodicon.2023.1344361
• EISSN: 1308-8084
• ISSN: 1308-5301

Neurodegeneration is an important finding of various neurological diseases such as Alzheimer's Disease and Parkinson's Disease. MAP2 and Rbfox3/NeuN genes are also two important neuronal markers. In this study, our aim is to investigate the neuroprotective effect of Cistus laurifolius L. extract in neurodegeneration caused by hydrogen peroxide (H2O2) treatment in differentiated SH-SY5Y (f-SH-SY5Y) cells. In the study, the effects of H2O2 (62.5-1000µM) and Cistus laurifolius (15.62-1000 mg/ml) doses on cell viability were determined by the 3-(4,5-dimethylthiazol-2-yl)2,5-diphenyl tetrasodium bromide (MTT) method. The effect of Cistus laurifolius in d-SH-SY5Y cells followed by H2O2treatment on cell viability was determined by the MTT test. The effect of the extract on MAP2 and Rbfox3/NeuN gene expressions in 24-hour periods was evaluated by the Real-Time Quantitative Reverse Transcription Polymerase Chain Reaction (RT-qPCR) method. According to our findings, Cistus laurifolius extract significantly reduced cell viability in SH-SY5Y cells at 24 h and 48 h (p<0.05). H2O2 caused a decrease in d-SH-SY5Y cell viability at all doses (p<0.05). We showed that Cistus laurifolius extract applied for 24 hours and 48 hours before toxicity significantly increased cell viability at doses of 31.25 and 62.5 mg/ml in 24 hours compared to the H2O2group. In d-SH-SY5Y cells, MAP2 gene expression in the H2O2 group was significantly decreased compared to the control group, however, it was upregulated in Cistus laurifolius groups at 31.25 and 62.5 mg/ml administered before toxicity compared to the H2O2group (p<0.05). However, there was no significant difference between the groups in terms of NeuN gene expression (p>0.05). The neuroprotective effect of Cistus laurifolius in the in-vitro neurodegeneration model has been extensively investigated for the first time. According to the data we obtained, Cistus laurifolius increased the cell viability and MAP2 gene expression and showed a neuroprotective effect. However, further studies are still needed to confirm the therapeutic value of the extract.