Antibacterial activity of Thymus vulgaris essential oil alone and in combination with cefotaxime against bla ESBL producing multidrug resistant Enterobacteriaceae isolates

• Published in: Natural product research Vol. 33; no. 18; pp. 2647 - 2654
• Main Authors: Benameur, Qada, Gervasi, Teresa, Pellizzeri, Vito, Pľuchtová, Mária, Tali-Maama, Hassiba, Assaous, Farida, Guettou, Badia, Rahal, Kheira, Gruľová, Daniela, Dugo, Giacomo, Marino, Andreana, Ben-Mahdi, Meriem-Hind
• Format: Journal Article
• Language: English
• Published: England 17-09-2019
• Subjects: ESBL; synergism; essential oil; antibacterial-resistance
• ISSN: 1478-6419; 1478-6427
• DOI: 10.1080/14786419.2018.1466124

The aim was to evaluate the susceptibility of
producing
to Slovakian
essential oil (TVEO) alone and in combination with cefotaxime (CTX). TVEO composition was determined by gas chromatograph-mass spectrometer (GC/MS). Susceptibility to 21 antibiotics was determined by disc diffusion assay. Genes characterization for resistance to β-lactams was accomplished by polymerase chain reaction (PCR). The antibacterial activity was investigated by standard methods. The synergistic interaction was determined by checkerboard test. Thymol (34.5%), p-cymene (22.27%) and linalool (5.35%) were the major components present in the TVEO. The identified strains were multi-drug resistant (MDR). TVEO showed high activity against all MDR strains, including
producing isolates, with inhibition zones and MIC values in the range of 24-40 mm/10μL and 2.87-11.5 μg/mL, respectively. TVEO in combination with CTX showed a synergistic action against
producing
(FICI 0.28) and an additive effect
ESBL producing
(FICI 0.987).