Cu 2+ -attached pumice particles embedded composite cryogels for protein purification

• Published in: Artificial cells, nanomedicine, and biotechnology Vol. 45; no. 1; p. 90
• Main Authors: Alkan, Hüseyin, Cömert, Şeyda Ceylan, Gürbüz, Fatma, Doğru, Mehmet, Odabaşı, Mehmet
• Format: Journal Article
• Language: English
• Published: England 01-02-2017
• Subjects: Copper - chemistry; Cryogels - chemistry; Humans; Serum Albumin - chemistry; Serum Albumin - isolation & purification; Silicates - chemistry; metal chelate; pumice; Composite cryogel; particle embedding; human serum albumin chromatography
• ISSN: 2169-141X
• DOI: 10.3109/21691401.2015.1129627

In this study, chromatographic performance of Cu
-attached pumice particles embedded to monolithic cryogels (Cu
-APPsEMC) for human serum albumin (HSA) was investigated. Monolithic composite cryogels were prepared by means of polymerization of gel-forming precursors at sub-zero temperatures. The chemical composition of pumice and surface of composite cryogels were determined by X-ray fluorescence spectrometer and scanning electron microscopy, respectively. The highest adsorption capacity (549.5 mg/g pumice) of cryogels was achieved at phosphate buffer of pH 8.0 with initial HSA solution of 3 mg/ml. SDS-PAGE analysis was performed for the samples studied on human serum to determine HSA adsorption/desorption performance of cryogel qualitatively.