The Role of Steroid Receptors in the Proliferation and Migration of Endometrial Adenocarcinoma HEC1A Cell Line
Objectives: Endometrial carcinoma is one of the most common gynecological cancers. It is generally divided into oestrogen-dependent type I, and oestrogen-independent type II. Although the expression of some steroid receptors has been documented in type II endometrial carcinoma, their roles in tumor...
Saved in:
| Published in: | Dicle tıp dergisi Vol. 50; no. 3; pp. 327 - 334 |
|---|---|
| Main Authors: | , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
Diyarbakir
Dicle University
15-09-2023
|
| Subjects: | |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Abstract | Objectives: Endometrial carcinoma is one of the most common gynecological cancers. It is generally divided into oestrogen-dependent type I, and oestrogen-independent type II. Although the expression of some steroid receptors has been documented in type II endometrial carcinoma, their roles in tumor progression have not been fully elucidated yet. Thus in this study, we aimed to examine the role of compounds acting on steroid receptors in type II, on HEC1A cultured cells.
Methods: We tested the effect of mifepristone (the glucocorticoid and progesterone receptor blocker, 10-8M), bicalutamide (the androgen receptor blocker, 10-6M), G15 (the G-protein-coupled estrogen receptor-1 blocker, 10-7M) and PHTPP (2-Phenyl-3-(4-hydroxyphenyl)-5,7-bis (trifluoromethyl)-pyrazolo [1,5-a]pyrimidine, the estrogen receptor-β blocker, 10-7M), on proliferation. Proliferation was assessed by xCELLigence analysis system and migration was examined by using wound-healing model.
Results: None of the drugs, at the used concentrations, have affected the proliferation of HEC1A cells. However, migration was significantly increased at the 24th and the 48th hour of mifepristone application (p |
|---|---|
| AbstractList | Objectives: Endometrial carcinoma is one of the most common gynecological cancers. It is generally divided into oestrogen-dependent type I, and oestrogen-independent type II. Although the expression of some steroid receptors has been documented in type II endometrial carcinoma, their roles in tumor progression have not been fully elucidated yet. Thus in this study, we aimed to examine the role of compounds acting on steroid receptors in type II, on HEC1A cultured cells.
Methods: We tested the effect of mifepristone (the glucocorticoid and progesterone receptor blocker, 10-8M), bicalutamide (the androgen receptor blocker, 10-6M), G15 (the G-protein-coupled estrogen receptor-1 blocker, 10-7M) and PHTPP (2-Phenyl-3-(4-hydroxyphenyl)-5,7-bis (trifluoromethyl)-pyrazolo [1,5-a]pyrimidine, the estrogen receptor-β blocker, 10-7M), on proliferation. Proliferation was assessed by xCELLigence analysis system and migration was examined by using wound-healing model.
Results: None of the drugs, at the used concentrations, have affected the proliferation of HEC1A cells. However, migration was significantly increased at the 24th and the 48th hour of mifepristone application (p [...]in this study, we aimed to examine the role of compounds acting on steroid receptors in type II, on HEC1A cultured cells. Genes regulated by steroid hormone receptors are essential for many biological activities including cell proliferation, survival, metabolism, and differentiation5'6. [...]impaired functioning of steroid receptors could lead to multiple forms of cancers such as breast, leukaemia and lymphoma, prostate, ovarian, and lung cancer6. Cells were subcultured every 7-10 days by sterile and standard techniques. Since the present work was conducted by a commercially available cell culture line; according to ethical rules in Turkey, there is no need for ethical approval. The effect of 10-7M PHTPP on proliferation The oestrogen receptor-ß blocker, PHTPP did not change cell proliferation at 10-7M when compared to the ethanol group (Fig. 4, p > 0.05, Steroid receptors blockers in migration The effect of 10-8M mifepristone on migration At a concentration of 10-8M, mifepristone significantly increased relative cell motility at both 24 and 48 hours when compared to the ethanol group (Fig. 5, p < 0.01, n=4). |
| Author | TİFTİK, R. Nalan BÜYÜKAFŞAR, Kansu ÜN, İsmail HENNAWİ, Khairat Al |
| Author_xml | – sequence: 1 givenname: Khairat Al surname: HENNAWİ fullname: HENNAWİ, Khairat Al – sequence: 2 givenname: R. Nalan surname: TİFTİK fullname: TİFTİK, R. Nalan – sequence: 3 givenname: İsmail surname: ÜN fullname: ÜN, İsmail – sequence: 4 givenname: Kansu surname: BÜYÜKAFŞAR fullname: BÜYÜKAFŞAR, Kansu |
| BookMark | eNo1kE1PAjEQhhuDiYjePTbxvNhuP2iPhKCYYDSI5023nWrJ0q7d5eC_dxE8zUzyzEze5xqNYoqA0B0lUzHT6sEF20Af2illkkjBLtCYMqIKrZQe_fWkKDUXV-i663aESMk1H6O4_QK8SQ3g5PF7DzkFhzdgoe1T7nCIuB-At5ya4CGbPqSITXT4JXyep2FvGV3aQ5-DafDcQUzWZBti2hu8Wi7oHC-gafA6RLhBl940Hdye6wR9PC63i1Wxfn16XszXhaWUsqKm0qvaWuFnqvaiVkCFdMxYKawqS4CSKwVKO20NKwW3nNQl4yC8FI5ZYBN0f7rb5vR9gK6vdumQ4_CyKtUxOuNMDxQ5UTanrsvgqzaHvck_FSXV0Wr1b7U6W2W_Ilxuyw |
| Cites_doi | 10.1200/JCO.2012.48.2596 10.2147/OTT.S169947 10.1530/JOE-13-0500 10.1530/ERC-13-0237 10.3727/096504017X15035795904677 10.1093/carcin/bgt348 10.1038/nrd1551 10.1186/s12885-019-5928-2 10.1186/s13046-015-0168-z 10.1016/j.ebiom.2019.08.035 10.2147/OTT.S182239 10.3390/ijms19041139 10.1021/jm049631k 10.1186/s12885-018-4030-5 10.1016/j.fertnstert.2005.01.126 10.1097/MD.0000000000019822 10.3892/ol.2017.6298 10.1038/s41598-019-50531-3 10.5798/dicletip.1128948 10.1038/s41419-021-03727-3 10.1038/pcan.2008.23 10.1007/s10552-010-9612-8 10.1038/emboj.2011.216 10.1016/j.canlet.2010.09.008 10.1371/journal.pone.0034672 10.18632/oncotarget.13471 10.18621/eurj.900847 10.1016/j.annonc.2020.06.011 |
| ContentType | Journal Article |
| Copyright | 2023. This work is published under https://creativecommons.org/licenses/by-nc/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. |
| Copyright_xml | – notice: 2023. This work is published under https://creativecommons.org/licenses/by-nc/4.0/ (the “License”). Notwithstanding the ProQuest Terms and Conditions, you may use this content in accordance with the terms of the License. |
| DBID | AAYXX CITATION 3V. 7X7 7XB 8FI 8FJ 8FK ABUWG AFKRA AZQEC BENPR CCPQU DWQXO EDSIH FYUFA GHDGH K9. M0S PIMPY PQEST PQQKQ PQUKI PRINS |
| DOI | 10.5798/dicletip.1360653 |
| DatabaseName | CrossRef ProQuest Central (Corporate) Health & Medical Collection ProQuest Central (purchase pre-March 2016) Hospital Premium Collection Hospital Premium Collection (Alumni Edition) ProQuest Central (Alumni) (purchase pre-March 2016) ProQuest Central (Alumni) ProQuest Central UK/Ireland ProQuest Central Essentials ProQuest Central ProQuest One Community College ProQuest Central Turkey Database Health Research Premium Collection Health Research Premium Collection (Alumni) ProQuest Health & Medical Complete (Alumni) Health & Medical Collection (Alumni Edition) Publicly Available Content (ProQuest) ProQuest One Academic Eastern Edition (DO NOT USE) ProQuest One Academic ProQuest One Academic UKI Edition ProQuest Central China |
| DatabaseTitle | CrossRef Publicly Available Content Database ProQuest Central Essentials ProQuest One Academic Eastern Edition ProQuest Health & Medical Complete (Alumni) ProQuest Central (Alumni Edition) ProQuest One Community College ProQuest Hospital Collection Health Research Premium Collection (Alumni) ProQuest Central China ProQuest Hospital Collection (Alumni) ProQuest Central ProQuest Health & Medical Complete Health Research Premium Collection ProQuest One Academic UKI Edition Health and Medicine Complete (Alumni Edition) ProQuest Central Korea Turkey Database ProQuest One Academic ProQuest Central (Alumni) |
| DatabaseTitleList | CrossRef Publicly Available Content Database |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Medicine |
| EISSN | 1308-9889 |
| EndPage | 334 |
| ExternalDocumentID | 10_5798_dicletip_1360653 |
| GeographicLocations | United States--US Turkey California |
| GeographicLocations_xml | – name: United States--US – name: California – name: Turkey |
| GroupedDBID | 29G 2WC 3V. 53G 7X7 8FI 8FJ 8R4 8R5 AAYXX ABDBF ABUWG ACIHN ADBBV AEAQA AFKRA AHMBA ALIPV ALMA_UNASSIGNED_HOLDINGS BAWUL BCNDV BENPR BPHCQ BVXVI CCPQU CITATION DIK EDSIH EOJEC ESX FYUFA GJB GROUPED_DOAJ GX1 HMCUK KQ8 MK0 OBODZ OK1 PIMPY PQQKQ PROAC Q2X RIG RNS SW3 TUS UKHRP 7XB 8FK AZQEC DWQXO K9. PQEST PQUKI PRINS |
| ID | FETCH-LOGICAL-c1113-b16f8bcc5f78bf5b8e156d3ac65c822ee2488e89d9ca3254c40b234e5f65d3ce3 |
| ISSN | 1300-2945 |
| IngestDate | Thu Oct 10 20:21:10 EDT 2024 Thu Nov 21 22:22:12 EST 2024 |
| IsDoiOpenAccess | true |
| IsOpenAccess | true |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 3 |
| Language | English |
| LinkModel | OpenURL |
| MergedId | FETCHMERGED-LOGICAL-c1113-b16f8bcc5f78bf5b8e156d3ac65c822ee2488e89d9ca3254c40b234e5f65d3ce3 |
| OpenAccessLink | https://www.proquest.com/docview/2866493439 |
| PQID | 2866493439 |
| PQPubID | 55023 |
| PageCount | 8 |
| ParticipantIDs | proquest_journals_2866493439 crossref_primary_10_5798_dicletip_1360653 |
| PublicationCentury | 2000 |
| PublicationDate | 2023-09-15 |
| PublicationDateYYYYMMDD | 2023-09-15 |
| PublicationDate_xml | – month: 09 year: 2023 text: 2023-09-15 day: 15 |
| PublicationDecade | 2020 |
| PublicationPlace | Diyarbakir |
| PublicationPlace_xml | – name: Diyarbakir |
| PublicationTitle | Dicle tıp dergisi |
| PublicationYear | 2023 |
| Publisher | Dicle University |
| Publisher_xml | – name: Dicle University |
| References | ref13 ref35 ref12 ref34 ref15 ref14 ref31 ref30 ref11 ref33 ref10 ref32 ref2 ref1 ref17 ref16 ref19 ref18 ref24 ref23 ref26 ref25 ref20 ref22 ref21 ref28 ref27 ref29 ref8 ref7 ref9 ref4 ref3 ref6 ref5 |
| References_xml | – ident: ref1 – ident: ref4 doi: 10.1200/JCO.2012.48.2596 – ident: ref12 doi: 10.2147/OTT.S169947 – ident: ref33 doi: 10.1530/JOE-13-0500 – ident: ref26 doi: 10.1530/ERC-13-0237 – ident: ref24 doi: 10.3727/096504017X15035795904677 – ident: ref29 doi: 10.1093/carcin/bgt348 – ident: ref25 – ident: ref5 doi: 10.1038/nrd1551 – ident: ref34 doi: 10.1186/s12885-019-5928-2 – ident: ref15 doi: 10.1186/s13046-015-0168-z – ident: ref13 doi: 10.1016/j.ebiom.2019.08.035 – ident: ref30 doi: 10.2147/OTT.S182239 – ident: ref9 doi: 10.3390/ijms19041139 – ident: ref28 doi: 10.1021/jm049631k – ident: ref32 doi: 10.1186/s12885-018-4030-5 – ident: ref11 doi: 10.1016/j.fertnstert.2005.01.126 – ident: ref17 – ident: ref20 doi: 10.1097/MD.0000000000019822 – ident: ref27 doi: 10.3892/ol.2017.6298 – ident: ref31 doi: 10.1038/s41598-019-50531-3 – ident: ref35 doi: 10.5798/dicletip.1128948 – ident: ref2 – ident: ref21 – ident: ref22 doi: 10.1038/s41419-021-03727-3 – ident: ref16 doi: 10.1038/pcan.2008.23 – ident: ref3 doi: 10.1007/s10552-010-9612-8 – ident: ref19 doi: 10.1038/emboj.2011.216 – ident: ref6 doi: 10.1016/j.canlet.2010.09.008 – ident: ref23 doi: 10.1371/journal.pone.0034672 – ident: ref8 – ident: ref18 – ident: ref7 doi: 10.18632/oncotarget.13471 – ident: ref10 doi: 10.18621/eurj.900847 – ident: ref14 doi: 10.1016/j.annonc.2020.06.011 |
| SSID | ssj0066494 ssib008506797 |
| Score | 2.283802 |
| Snippet | Objectives: Endometrial carcinoma is one of the most common gynecological cancers. It is generally divided into oestrogen-dependent type I, and... [...]in this study, we aimed to examine the role of compounds acting on steroid receptors in type II, on HEC1A cultured cells. Genes regulated by steroid... |
| SourceID | proquest crossref |
| SourceType | Aggregation Database |
| StartPage | 327 |
| SubjectTerms | Androgens Cell adhesion & migration Cell culture Cell growth Endometrial cancer Estrogens Ethanol Gynecology Hormone replacement therapy Motility Ovaries Steroids Wound healing |
| Title | The Role of Steroid Receptors in the Proliferation and Migration of Endometrial Adenocarcinoma HEC1A Cell Line |
| URI | https://www.proquest.com/docview/2866493439 |
| Volume | 50 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://sdu.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtV1Lb9NAEF6lRUJcKp6itKA90AOKDLbXrz0mjqNWLVXVFImbZe-jWFA7SlIh_j0z3rWd9IDgwMVJ1ort7HyZmZ39ZoaQ96UKmfQ1d2SRcAfWG8opGDJzokKARS80T9omtov48msyy4JsNOq6gw5j_1XSMAayxszZf5B2f1EYgPcgcziC1OH413K_tpTBBUxaU2ECIpJXsK2OZTVeYa8eraz0W65Fdbvqvcesls2dMg09JqCYwN6tRFU3mLaUpd5knGLE76Lbkre-7QwfZbxB93TqLcdSrTAo0iMKixjXdfHT0Ae-FbjB38cNKr2pWr18Dfr-x4BY3MfnqclOW99t8UGmeGaa_jIv3wt9koYnfF5Yikht-rl08QyfIfnCZHQaUkr7rAMrZUs3M9d1fG6qT35U3Rjo68R0IeoUuqlka4HLtrQzM2UIrKFnJor60IaEMce8CInPsamWyAPE-r2Dvew4AqeTRX41m-cXZ5fne-SRD5quXdOfnfcKDcsBxryPAERRwE2XZftLzL453vHTw_vt-km7bkLr-9w8JQd20UInBm3PyEjVz8njz5aW8YLUADqKoKONphZ0tAcdrWoKoKM7oKMAOtqDDr-3BTq6Czrago4i6CiC7iX5Ms9u0lPHNvJwBJhS5pRepJNSiFDHSanDMlFeGElWiCgU4KAq5YMZUQmXXBTMDwMRuKXPAhXqKJRMKPaK7NdNrV4TynwpdOyKtsK6TMoykPABvHDluQo0yyH50E1bvjT1WnJY5-IU590U53aKD8lxN6-5_Qevcz9BKTHw09_8-fQReTLA95jsb1b36i3ZW8v7dy0IfgNGdI_S |
| link.rule.ids | 315,782,786,27933,27934 |
| linkProvider | Flying Publisher |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=The+Role+of+Steroid+Receptors+in+the+Proliferation+and+Migration+of+Endometrial+Adenocarcinoma+HEC1A+Cell+Line&rft.jtitle=Dicle+t%C4%B1p+dergisi&rft.au=Al+Hennawi%2C+Khairat&rft.au=Tiftik%2C+R+Nalan&rft.au=%C3%9Cn%2C+Ismail&rft.au=B%C3%BCy%C3%BCkaf%C5%9Far%2C+Kansu&rft.date=2023-09-15&rft.pub=Dicle+University&rft.issn=1300-2945&rft.eissn=1308-9889&rft.volume=50&rft.issue=3&rft.spage=327&rft.epage=334&rft_id=info:doi/10.5798%2Fdicletip.1360653&rft.externalDBID=HAS_PDF_LINK |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1300-2945&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1300-2945&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1300-2945&client=summon |