In silico evolutionary analysis of Helicobacter pylori outer membrane phospholipase A (OMPLA)

• Published in: BMC microbiology Vol. 12; no. 1; p. 206
• Main Authors: Vollan, Hilde S, Tannaes, Tone, Yamaoka, Yoshio, Bukholm, Geir
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 13-09-2012; BioMed Central; BMC
• Subjects: Adaptation; Analysis; Bacterial Outer Membrane Proteins - genetics; Biogeography; Codon; Computational Biology; Evolution; Evolution, Molecular; Genes; Genetic aspects; Helicobacter pylori; Helicobacter pylori - enzymology; Helicobacter pylori - genetics; Microbiology; Molecular evolution; Molecular Sequence Data; OMPLA; Outer membrane; Phospholipase A; Phospholipases; Phospholipases A1 - genetics; Phylogeny; Physiological aspects; pldA; Proteins; Sequence Analysis, DNA; Ulcers; Norway; United States
• ISSN: 1471-2180; 1471-2180
• DOI: 10.1186/1471-2180-12-206

In the past decade, researchers have proposed that the pldA gene for outer membrane phospholipase A (OMPLA) is important for bacterial colonization of the human gastric ventricle. Several conserved Helicobacter pylori genes have distinct genotypes in different parts of the world, biogeographic patterns that can be analyzed through phylogenetic trees. The current study will shed light on the importance of the pldA gene in H. pylori. In silico sequence analysis will be used to investigate whether the bacteria are in the process of preserving, optimizing, or rejecting the pldA gene. The pldA gene will be phylogenetically compared to other housekeeping (HK) genes, and a possible origin via horizontal gene transfer (HGT) will be evaluated through both intra- and inter-species evolutionary analyses.
In this study, pldA gene sequences were phylogenetically analyzed and compared with a large reference set of concatenated HK gene sequences. A total of 246 pldA nucleotide sequences were used; 207 were from Norwegian isolates, 20 were from Korean isolates, and 19 were from the NCBI database. Best-fit evolutionary models were determined with MEGA5 ModelTest for the pldA (K80 + I + G) and HK (GTR + I + G) sequences, and maximum likelihood trees were constructed. Both HK and pldA genes showed biogeographic clustering. Horizontal gene transfer was inferred based on significantly different GC contents, the codon adaptation index, and a phylogenetic conflict between a tree of OMPLA protein sequences representing 171 species and a tree of the AtpA HK protein for 169 species. Although a vast majority of the residues in OMPLA were predicted to be under purifying selection, sites undergoing positive selection were also found.
Our findings indicate that the pldA gene could have been more recently acquired than seven of the HK genes found in H. pylori. However, the common biogeographic patterns of both the HK and pldA sequences indicated that the transfer occurred long ago. Our results indicate that the bacterium is preserving the function of OMPLA, although some sites are still being evolutionarily optimized.