Detection of dengue virus in saliva and urine by real time RT-PCR

• Published in: Virology journal Vol. 7; no. 1; p. 22
• Main Authors: Poloni, Telma R, Oliveira, Anibal S, Alfonso, Helda L, Galvão, Larissa R, Amarilla, Alberto A, Poloni, Dimair F, Figueiredo, Luiz T, Aquino, Victor H
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 27-01-2010; BioMed Central; BMC
• Subjects: Adolescent; Adult; Brazil; Dengue - diagnosis; Dengue fever; Dengue Virus - isolation & purification; Dengue viruses; Female; Fever; Gene amplification; Hospitals; Humans; Identification and classification; Methods; Phylogenetics; Polymerase chain reaction; Properties; Reverse transcriptase; Reverse Transcriptase Polymerase Chain Reaction - methods; RNA, Viral - genetics; RNA, Viral - isolation & purification; Saliva; Saliva - virology; Salivary glands; secretions; Serum - virology; Short Report; Urine; Urine - virology; Viruses; Brazil
• ISSN: 1743-422X; 1743-422X
• DOI: 10.1186/1743-422x-7-22

Early diagnosis of dengue virus (DENV) infection is important for patient management and control of dengue outbreaks. The objective of this study was to analyze the usefulness of urine and saliva samples for early diagnosis of DENV infection by real time RT-PCR. Two febrile patients, who have been attended at the General Hospital of the School of Medicine of Ribeirao Preto, Sao Paulo University were included in the study. Serum, urine and saliva samples collected from both patients were subjected to real time RT-PCR for DENV detection and quantification. Dengue RNA was detected in serum, urine and saliva samples of both patients. Patient 1 was infected with DENV-2 and patient 2 with DENV-3. Data presented in this study suggest that urine and saliva could be used as alternative samples for early diagnosis of dengue virus infection when blood samples are difficult to obtain, e.g., in newborns and patients with hemorrhagic syndromes.