Genome-wide mapping of NBS-LRR genes and their association with disease resistance in soybean

• Published in: BMC plant biology Vol. 12; no. 1; p. 139
• Main Authors: Kang, Yang Jae, Kim, Kil Hyun, Shim, Sangrea, Yoon, Min Young, Sun, Suli, Kim, Moon Young, Van, Kyujung, Lee, Suk-Ha
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 09-08-2012; BioMed Central; BMC
• Subjects: Analysis; Bacteria; Bacterial infections; Base Sequence; Binding Sites; Chromosome Mapping; Chromosomes; Chromosomes, Plant - genetics; Disease; Disease Resistance; Disease susceptibility; Drug resistance in microorganisms; Gene Duplication; Gene expression; Gene Expression Profiling; Genes; Genes, Plant; Genetic aspects; Genetic research; Genome duplication; Genomes; Genomics; Glycine max; Glycine max - genetics; Glycine max - immunology; Glycine max - microbiology; Leucine-Rich Repeat Proteins; Life sciences; Medical research; Medicine, Experimental; Multigene Family; NBS-LRR; Physiological aspects; Plant Diseases - immunology; Plant Diseases - microbiology; Plant genetics; Plant Immunity - genetics; Plant immunology; Plant Leaves - immunology; Plant Leaves - microbiology; Protein binding; Proteins; Proteins - genetics; Proteins - metabolism; Quantitative genetics; Quantitative Trait Loci; Rural development; Software; Soybean; Transcriptome; Transcriptome analysis; Xanthomonas axonopodis; Xanthomonas axonopodis - immunology; Xanthomonas axonopodis - pathogenicity; South Korea
• ISSN: 1471-2229; 1471-2229
• DOI: 10.1186/1471-2229-12-139

R genes are a key component of genetic interactions between plants and biotrophic bacteria and are known to regulate resistance against bacterial invasion. The most common R proteins contain a nucleotide-binding site and a leucine-rich repeat (NBS-LRR) domain. Some NBS-LRR genes in the soybean genome have also been reported to function in disease resistance. In this study, the number of NBS-LRR genes was found to correlate with the number of disease resistance quantitative trait loci (QTL) that flank these genes in each chromosome. NBS-LRR genes co-localized with disease resistance QTL. The study also addressed the functional redundancy of disease resistance on recently duplicated regions that harbor NBS-LRR genes and NBS-LRR gene expression in the bacterial leaf pustule (BLP)-induced soybean transcriptome.
A total of 319 genes were determined to be putative NBS-LRR genes in the soybean genome. The number of NBS-LRR genes on each chromosome was highly correlated with the number of disease resistance QTL in the 2-Mb flanking regions of NBS-LRR genes. In addition, the recently duplicated regions contained duplicated NBS-LRR genes and duplicated disease resistance QTL, and possessed either an uneven or even number of NBS-LRR genes on each side. The significant difference in NBS-LRR gene expression between a resistant near-isogenic line (NIL) and a susceptible NIL after inoculation of Xanthomonas axonopodis pv. glycines supports the conjecture that NBS-LRR genes have disease resistance functions in the soybean genome.
The number of NBS-LRR genes and disease resistance QTL in the 2-Mb flanking regions of each chromosome was significantly correlated, and several recently duplicated regions that contain NBS-LRR genes harbored disease resistance QTL for both sides. In addition, NBS-LRR gene expression was significantly different between the BLP-resistant NIL and the BLP-susceptible NIL in response to bacterial infection. From these observations, NBS-LRR genes are suggested to contribute to disease resistance in soybean. Moreover, we propose models for how NBS-LRR genes were duplicated, and apply Ks values for each NBS-LRR gene cluster.