Transcript copy number estimation using a mouse whole-genome oligonucleotide microarray

• Published in: Genome biology Vol. 6; no. 7; p. R61
• Main Authors: Carter, Mark G, Sharov, Alexei A, VanBuren, Vincent, Dudekula, Dawood B, Carmack, Condie E, Nelson, Charlie, Ko, Minoru S H
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 01-01-2005; BioMed Central
• Subjects: Animals; Base Sequence; Expressed Sequence Tags; Gene Expression Profiling; Genome; Introns; Method; Mice; Models, Genetic; Oligonucleotide Array Sequence Analysis; Oligonucleotide Probes; Open Reading Frames; Restriction Mapping; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - genetics; Saccharomyces cerevisiae - genetics; Transcription, Genetic
• ISSN: 1474-760X; 1465-6906; 1474-760X; 1465-6914
• DOI: 10.1186/gb-2005-6-7-r61

The ability to quantitatively measure the expression of all genes in a given tissue or cell with a single assay is an exciting promise of gene-expression profiling technology. An in situ-synthesized 60-mer oligonucleotide microarray designed to detect transcripts from all mouse genes was validated, as well as a set of exogenous RNA controls derived from the yeast genome (made freely available without restriction), which allow quantitative estimation of absolute endogenous transcript abundance.