Induction of apoptosis in A549 pulmonary cells by two Paracoccidioides brasiliensis samples

Paracoccidioidomycosis presents a variety of clinical manifestations and Paracoccidioides brasiliensis can reach many tissues, most importantly the lungs. The ability of the pathogen to interact with host surface structures is essential to its virulence. The interaction between P. brasiliensis and e...

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Published in:	Memórias do Instituto Oswaldo Cruz Vol. 104; no. 5; pp. 749 - 754
Main Authors:	Del Vecchio, Adriana, da Silva, Julhiany de Fatima, da Silva, Juliana Leal Monteiro, Andreotti, Patricia Ferrari, Soares, Christiane Pienna, Benard, Gil, Giannini, Maria José Soares Mendes
Format:	Journal Article
Language:	English
Published:	Brazil Fundação Oswaldo Cruz, Fiocruz 01-08-2009 Instituto Oswaldo Cruz, Ministério da Saúde Fundação Oswaldo Cruz (FIOCRUZ)
Subjects:	apoptosis apoptosis - Paracoccidioides brasiliensis - caspase 3 - Bak - Bcl-2 Apoptosis - physiology Bak Bcl-2 bcl-2 Homologous Antagonist-Killer Protein - analysis caspase 3 Caspase 3 - analysis Cell Line - microbiology Epithelial Cells - microbiology Flow Cytometry Host-Pathogen Interactions Humans Lung - cytology Paracoccidioides - pathogenicity Paracoccidioides - physiology Paracoccidioides brasiliensis PARASITOLOGY Proto-Oncogene Proteins c-bcl-2 - analysis TROPICAL MEDICINE Bcl-2 apoptosis caspase 3 Bak Paracoccidioides brasiliensis
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Abstract	Paracoccidioidomycosis presents a variety of clinical manifestations and Paracoccidioides brasiliensis can reach many tissues, most importantly the lungs. The ability of the pathogen to interact with host surface structures is essential to its virulence. The interaction between P. brasiliensis and epithelial cells has been studied, with particular emphasis on the induction of apoptosis. To investigate the expression of different apoptosis-inducing pathways in human A549 cells, we infected these cells with P. brasiliensis Pb18SP (subcultured) and 18R (recently isolated from cell culture and showing a high adhesion pattern) samples in vitro. The expressions of Bcl-2, Bak and caspase 3 were analysed by flow cytometry and DNA fragmentation using the TUNEL technique. Apoptosis of human A549 cells was induced by P. brasiliensis in a sample and time-dependent manner. Using an in vitro model, our data demonstrates that caspase 3, Bak, Bcl-2 and DNA fragmentation mediate P. brasiliensis-induced apoptosis in A549 cells. The overall mechanism is a complex process, which may involve several signal transduction pathways. These findings could partially explain the efficient behaviour of this fungus in promoting tissue infection and/or blood dissemination.
AbstractList	Paracoccidioidomycosis presents a variety of clinical manifestations and Paracoccidioides brasiliensis can reach many tissues, most importantly the lungs. The ability of the pathogen to interact with host surface structures is essential to its virulence. The interaction between P. brasiliensis and epithelial cells has been studied, with particular emphasis on the induction of apoptosis. To investigate the expression of different apoptosis-inducing pathways in human A549 cells, we infected these cells with P. brasiliensis Pb18SP (subcultured) and 18R (recently isolated from cell culture and showing a high adhesion pattern) samples in vitro. The expressions of Bcl-2, Bak and caspase 3 were analysed by flow cytometry and DNA fragmentation using the TUNEL technique. Apoptosis of human A549 cells was induced by P. brasiliensis in a sample and time-dependent manner. Using an in vitro model, our data demonstrates that caspase 3, Bak, Bcl-2 and DNA fragmentation mediate P. brasiliensis-induced apoptosis in A549 cells. The overall mechanism is a complex process, which may involve several signal transduction pathways. These findings could partially explain the efficient behaviour of this fungus in promoting tissue infection and/or blood dissemination. Parococcidioidomycosis presents a variety of clinical manifestations and Paracoccidioides brasiliensis can reach many tissues, most importantly the lungs. The ability of the pathogen to interact with host surface structures is essential to its virulence. The interaction between P. brasiliensis and epithelial cells has been studied, with particular emphasis on the induction of apoptosis. To investigate the expression of different apoptosis-inducing pathways in human A549 cells, we infected these cells with P. brasiliensis Pb18SP (subcultured) and 18R (recently isolated from cell culture and showing a high adhesion pattern) samples in vitro. The expressions of Bcl-2, Bak and caspase 3 were analysed by flow cytometry and DNA fragmentation using the TUNEL technique. Apoptosis of human A549 cells was induced by P. brasiliensis in a sample and time-dependent manner. Using an in vitro model, our data demonstrates that caspase 3, Bak, Bcl-2 and DNA fragmentation mediate P. brasiliensis-induced apoptosis in A549 cells. The overall mechanism is a complex process, which may involve several signal transduction pathways. These findings could partially explain the efficient behaviour of this fungus in promoting tissue infection and/or blood dissemination. Paracoccidioidomycosis presents a variety of clinical manifestations and Paracoccidioides brasiliensis can reach many tissues, most importantly the lungs. The ability of the pathogen to interact with host surface structures is essential to its virulence. The interaction between P. brasiliensis and epithelial cells has been studied, with particular emphasis on the induction of apoptosis. To investigate the expression of different apoptosis-inducing pathways in human A549 cells, we infected these cells with P. brasiliensis Pb18SP (subcultured) and 18R (recently isolated from cell culture and showing a high adhesion pattern) samples in vitro. The expressions of Bcl-2, Bak and caspase 3 were analysed by flow cytometry and DNA fragmentation using the TUNEL technique. Apoptosis of human A549 cells was induced by P. brasiliensis in a sample and time-dependent manner. Using an in vitro model, our data demonstrates that caspase 3, Bak, Bcl-2 and DNA fragmentation mediate P. brasiliensis-induced apoptosis in A549 cells. The overall mechanism is a complex process, which may involve several signal transduction pathways. These findings could partially explain the efficient behaviour of this fungus in promoting tissue infection and/or blood dissemination.
Author	Del Vecchio, Adriana Giannini, Maria José Soares Mendes Andreotti, Patricia Ferrari da Silva, Julhiany de Fatima Soares, Christiane Pienna da Silva, Juliana Leal Monteiro Benard, Gil
AuthorAffiliation	Universidade Estadual Paulista Universidade de São Paulo Instituto de Medicina Tropical
AuthorAffiliation_xml	– name: Universidade de São Paulo – name: Instituto de Medicina Tropical – name: Universidade Estadual Paulista
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BackLink	https://www.ncbi.nlm.nih.gov/pubmed/19820837$$D View this record in MEDLINE/PubMed
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Cites_doi	10.1016/S0074-7696(08)62419-5 10.1172/JCI119557 10.1080/mmy.38.s1.113.123 10.1126/science.281.5381.1322 10.1016/S0960-9822(99)80068-2 10.1016/j.femsim.2005.05.014 10.1007/s11046-007-9074-z 10.1590/S0074-02762006000300019 10.1006/clim.2002.5272 10.4049/jimmunol.168.12.6358 10.1083/jcb.119.3.493 10.1016/j.micinf.2005.02.005 10.1006/excr.1998.4172 10.1074/jbc.273.11.6121 10.1016/S0092-8674(00)80434-1 10.1126/science.7878464 10.1080/02681218780000461 10.1080/00362177785380121 10.1016/j.micinf.2004.05.005 10.1007/s00430-003-0180-3 10.1086/374646 10.1016/S1074-7613(00)80062-X 10.1016/S1286-4579(00)00390-7 10.1007/s10482-006-9129-z 10.1016/j.micinf.2007.09.018 10.1038/20959 10.1016/j.micinf.2006.08.012 10.1074/jbc.273.51.34272 10.1007/BF00437001 10.1080/02681219480000251
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Keywords	Bcl-2 apoptosis caspase 3 Bak Paracoccidioides brasiliensis
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Snippet	Paracoccidioidomycosis presents a variety of clinical manifestations and Paracoccidioides brasiliensis can reach many tissues, most importantly the lungs. The... Paracoccidioidomycosis presents a variety of clinical manifestations and Paracoccidioides brasiliensis can reach many tissues, most importantly the lungs. The... Parococcidioidomycosis presents a variety of clinical manifestations and Paracoccidioides brasiliensis can reach many tissues, most importantly the lungs. The...
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SubjectTerms	apoptosis apoptosis - Paracoccidioides brasiliensis - caspase 3 - Bak - Bcl-2 Apoptosis - physiology Bak Bcl-2 bcl-2 Homologous Antagonist-Killer Protein - analysis caspase 3 Caspase 3 - analysis Cell Line - microbiology Epithelial Cells - microbiology Flow Cytometry Host-Pathogen Interactions Humans Lung - cytology Paracoccidioides - pathogenicity Paracoccidioides - physiology Paracoccidioides brasiliensis PARASITOLOGY Proto-Oncogene Proteins c-bcl-2 - analysis TROPICAL MEDICINE
Title	Induction of apoptosis in A549 pulmonary cells by two Paracoccidioides brasiliensis samples
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