Urinary nitrate excretion is increased in patients with rheumatoid arthritis and reduced by prednisolone

• Published in: Annals of the rheumatic diseases Vol. 54; no. 10; pp. 820 - 824
• Main Authors: Stichtenoth, D O, Fauler, J, Zeidler, H, Frölich, J C
• Format: Journal Article
• Language: English
• Published: London BMJ Publishing Group Ltd and European League Against Rheumatism 01-10-1995; BMJ; BMJ Publishing Group LTD
• Subjects: Adult; Aged; Anti-Inflammatory Agents - therapeutic use; Arthritis, Rheumatoid - blood; Arthritis, Rheumatoid - drug therapy; Arthritis, Rheumatoid - urine; Biological and medical sciences; Blood Sedimentation; C-Reactive Protein - metabolism; Chromatography, Gas; Diseases of the osteoarticular system; Female; Humans; Inflammatory joint diseases; Male; Medical sciences; Middle Aged; Nitrates - urine; Nitric Oxide - biosynthesis; Prednisolone - therapeutic use; Severity of Illness Index; Human; Urine; Immunopathology; Corticosteroid; Chronic; Rheumatoid arthritis; Diseases of the osteoarticular system; Nitrogen monoxide; Autoimmune disease; Exploration; Inflammatory joint disease; Prednisolone
• ISSN: 0003-4967; 1468-2060
• DOI: 10.1136/ard.54.10.820

OBJECTIVES--To determine daily production of nitric oxide (NO) measured as urinary nitrate excretion, and the effect of prednisolone in patients with rheumatoid arthritis (RA). METHODS--Twenty four hour urinary nitrate was measured by gas chromatography in 10 patients with RA, before and two to four weeks after commencement of prednisolone 0.5 mg/kg body weight, and in 18 healthy controls. RESULTS--Before the start of prednisolone treatment the urinary nitrate excretion in patients with RA was 2.7-fold greater (p < 0.001) than that in healthy volunteers. After prednisolone it decreased significantly, by 28%, at which time inflammatory activity (as indicated by C reactive protein, erythrocyte sedimentation rate, joint count, and early morning stiffness) was also reduced considerably. Despite this decrease, the urinary nitrate excretion in patients with RA remained twice that in the control group (p < 0.05). CONCLUSIONS--Our data suggest that the endogenous production of NO is enhanced in patients with RA. Furthermore, the results indicate that, in parallel with suppression of inflammation, this increased NO synthesis could be reduced by prednisolone treatment.