Characterisation of divergent flavivirus NS3 and NS5 protein sequences detected in Rhipicephalus microplus ticks from Brazil

Characterisation of divergent flavivirus NS3 and NS5 protein sequences detected in Rhipicephalus microplus ticks from Brazil

Transcripts similar to those that encode the nonstructural (NS) proteins NS3 and NS5 from flaviviruses were found in a salivary gland (SG) complementary DNA (cDNA) library from the cattle tick Rhipicephalus microplus . Tick extracts were cultured with cells to enable the isolation of viruses capable...

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Published in:Memórias do Instituto Oswaldo Cruz Vol. 109; no. 1; pp. 38 - 50
Main Authors: Maruyama, Sandra Regina, Castro-Jorge, Luiza Antunes, Chaves Ribeiro, José Marcos, Gardinassi, Luiz Gustavo, Garcia, Gustavo Rocha, Giampietro Brandão, Lucinda, Rezende Rodrigues, Aline, Ituo Okada, Marcos, Pereira Abrão, Emiliana, Ferreira, Beatriz Rossetti, de Fonseca, Benedito Antonio Lopes, de Miranda-Santos, Isabel Kinney Ferreira
Format: Journal Article
Language:English
Published: Brazil Fundação Oswaldo Cruz, Fiocruz 01-02-2014
Instituto Oswaldo Cruz, Ministério da Saúde
Fundação Oswaldo Cruz (FIOCRUZ)
Subjects:
Animals
Brazil
Cattle
Conserved Sequence - genetics
flavivirus
Flavivirus - chemistry
Flavivirus - classification
Flavivirus - isolation & purification
Gene Library
Hydrophobic and Hydrophilic Interactions
NS3
NS5
PARASITOLOGY
Phylogeny
Polymerase Chain Reaction
Rhipicephalus - virology
Rhipicephalus microplus
RNA Helicases - chemistry
RNA, Viral - isolation & purification
Sequence Alignment - statistics & numerical data
Sequence Analysis, Protein - methods
Serine Endopeptidases - chemistry
tick
Tissue Extracts - analysis
Transcriptome - genetics
TROPICAL MEDICINE
Viral Nonstructural Proteins - chemistry
Brazil
NS5
tick
Rhipicephalus microplus
NS3
flavivirus
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Abstract Transcripts similar to those that encode the nonstructural (NS) proteins NS3 and NS5 from flaviviruses were found in a salivary gland (SG) complementary DNA (cDNA) library from the cattle tick Rhipicephalus microplus . Tick extracts were cultured with cells to enable the isolation of viruses capable of replicating in cultured invertebrate and vertebrate cells. Deep sequencing of the viral RNA isolated from culture supernatants provided the complete coding sequences for the NS3 and NS5 proteins and their molecular characterisation confirmed similarity with the NS3 and NS5 sequences from other flaviviruses. Despite this similarity, phylogenetic analyses revealed that this potentially novel virus may be a highly divergent member of the genus Flavivirus. Interestingly, we detected the divergent NS3 and NS5 sequences in ticks collected from several dairy farms widely distributed throughout three regions of Brazil. This is the first report of flavivirus-like transcripts in R. microplus ticks. This novel virus is a potential arbovirus because it replicated in arthropod and mammalian cells; furthermore, it was detected in a cDNA library from tick SGs and therefore may be present in tick saliva. It is important to determine whether and by what means this potential virus is transmissible and to monitor the virus as a potential emerging tick-borne zoonotic pathogen.
AbstractList Transcripts similar to those that encode the nonstructural (NS) proteins NS3 and NS5 from flaviviruses were found in a salivary gland (SG) complementary DNA (cDNA) library from the cattle tick Rhipicephalus microplus. Tick extracts were cultured with cells to enable the isolation of viruses capable of replicating in cultured invertebrate and vertebrate cells. Deep sequencing of the viral RNA isolated from culture supernatants provided the complete coding sequences for the NS3 and NS5 proteins and their molecular characterisation confirmed similarity with the NS3 and NS5 sequences from other flaviviruses. Despite this similarity, phylogenetic analyses revealed that this potentially novel virus may be a highly divergent member of the genus Flavivirus. Interestingly, we detected the divergent NS3 and NS5 sequences in ticks collected from several dairy farms widely distributed throughout three regions of Brazil. This is the first report of flavivirus-like transcripts in R. microplus ticks. This novel virus is a potential arbovirus because it replicated in arthropod and mammalian cells; furthermore, it was detected in a cDNA library from tick SGs and therefore may be present in tick saliva. It is important to determine whether and by what means this potential virus is transmissible and to monitor the virus as a potential emerging tick-borne zoonotic pathogen.
Transcripts similar to those that encode the nonstructural (NS) proteins NS3 and NS5 from flaviviruses were found in a salivary gland (SG) complementary DNA (cDNA) library from the cattle tick Rhipicephalus microplus. Tick extracts were cultured with cells to enable the isolation of viruses capable of replicating in cultured invertebrate and vertebrate cells. Deep sequencing of the viral RNA isolated from culture supernatants provided the complete coding sequences for the NS3 and NS5 proteins and their molecular characterisation confirmed similarity with the NS3 and NS5 sequences from other flaviviruses. Despite this similarity, phylogenetic analyses revealed that this potentially novel virus may be a highly divergent member of the genus Flavivirus. Interestingly, we detected the divergent NS3 and NS5 sequences in ticks collected from several dairy farms widely distributed throughout three regions of Brazil. This is the first report of flavivirus-like transcripts in R. microplus ticks. This novel virus is a potential arbovirus because it replicated in arthropod and mammalian cells; furthermore, it was detected in a cDNA library from tick SGs and therefore may be present in tick saliva. It is important to determine whether and by what means this potential virus is transmissible and to monitor the virus as a potential emerging tick-borne zoonotic pathogen.
Transcripts similar to those that encode the nonstructural (NS) proteins NS3 and NS5 from flaviviruses were found in a salivary gland (SG) complementary DNA (cDNA) library from the cattle tick Rhipicephalus microplus . Tick extracts were cultured with cells to enable the isolation of viruses capable of replicating in cultured invertebrate and vertebrate cells. Deep sequencing of the viral RNA isolated from culture supernatants provided the complete coding sequences for the NS3 and NS5 proteins and their molecular characterisation confirmed similarity with the NS3 and NS5 sequences from other flaviviruses. Despite this similarity, phylogenetic analyses revealed that this potentially novel virus may be a highly divergent member of the genus Flavivirus. Interestingly, we detected the divergent NS3 and NS5 sequences in ticks collected from several dairy farms widely distributed throughout three regions of Brazil. This is the first report of flavivirus-like transcripts in R. microplus ticks. This novel virus is a potential arbovirus because it replicated in arthropod and mammalian cells; furthermore, it was detected in a cDNA library from tick SGs and therefore may be present in tick saliva. It is important to determine whether and by what means this potential virus is transmissible and to monitor the virus as a potential emerging tick-borne zoonotic pathogen.
Transcripts similar to those that encode the nonstructural (NS) proteins NS3 and NS5 from flaviviruses were found in a salivary gland (SG) complementary DNA (cDNA) library from the cattle tick Rhipicephalus microplus.Tick extracts were cultured with cells to enable the isolation of viruses capable of replicating in cultured invertebrate and vertebrate cells. Deep sequencing of the viral RNA isolated from culture supernatants provided the complete coding sequences for the NS3 and NS5 proteins and their molecular characterisation confirmed similarity with the NS3 and NS5 sequences from other flaviviruses. Despite this similarity, phylogenetic analyses revealed that this potentially novel virus may be a highly divergent member of the genus Flavivirus. Interestingly, we detected the divergent NS3 and NS5 sequences in ticks collected from several dairy farms widely distributed throughout three regions of Brazil. This is the first report of flavivirus-like transcripts inR. microplus ticks. This novel virus is a potential arbovirus because it replicated in arthropod and mammalian cells; furthermore, it was detected in a cDNA library from tick SGs and therefore may be present in tick saliva. It is important to determine whether and by what means this potential virus is transmissible and to monitor the virus as a potential emerging tick-borne zoonotic pathogen.
Author Garcia, Gustavo Rocha
Pereira Abrão, Emiliana
Ferreira, Beatriz Rossetti
de Fonseca, Benedito Antonio Lopes
Gardinassi, Luiz Gustavo
Giampietro Brandão, Lucinda
Castro-Jorge, Luiza Antunes
Rezende Rodrigues, Aline
de Miranda-Santos, Isabel Kinney Ferreira
Chaves Ribeiro, José Marcos
Ituo Okada, Marcos
Maruyama, Sandra Regina
AuthorAffiliation 5 Laticínios Vale dos Buritis, Buritis, MG, Brasil
3 Faculdade de Tecnologia, Araçatuba, SP, Brasil
7 Universidade de São Paulo, Escola de Enfermagem de Ribeirão Preto, Ribeirão Preto, SP, Brasil, Escola de Enfermagem de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brasil
2 National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD, USA
4 Vallée SA, São Paulo, SP, Brasil
6 Vetmais Medicina Animal, Campinas, SP, Brasil
1 Faculdade de Medicina de Ribeirão Preto
AuthorAffiliation_xml – name: 4 Vallée SA, São Paulo, SP, Brasil
– name: 1 Faculdade de Medicina de Ribeirão Preto
– name: 6 Vetmais Medicina Animal, Campinas, SP, Brasil
– name: 3 Faculdade de Tecnologia, Araçatuba, SP, Brasil
– name: 7 Universidade de São Paulo, Escola de Enfermagem de Ribeirão Preto, Ribeirão Preto, SP, Brasil, Escola de Enfermagem de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brasil
– name: 5 Laticínios Vale dos Buritis, Buritis, MG, Brasil
– name: 2 National Institute of Allergy and Infectious Diseases, National Institutes of Health, Rockville, MD, USA
– name: Universidade de São Paulo
– name: Laticínios Vale dos Buriti
– name: National Institutes of Health
– name: Vetmais Medicina Animal
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Author_xml – sequence: 1
  fullname: Maruyama, Sandra Regina
– sequence: 2
  fullname: Castro-Jorge, Luiza Antunes
– sequence: 3
  fullname: Chaves Ribeiro, José Marcos
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BackLink https://www.ncbi.nlm.nih.gov/pubmed/24626302$$D View this record in MEDLINE/PubMed
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Issue 1
Keywords NS5
tick
Rhipicephalus microplus
NS3
flavivirus
Language English
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Snippet Transcripts similar to those that encode the nonstructural (NS) proteins NS3 and NS5 from flaviviruses were found in a salivary gland (SG) complementary DNA...
Transcripts similar to those that encode the nonstructural (NS) proteins NS3 and NS5 from flaviviruses were found in a salivary gland (SG) complementary DNA...
Transcripts similar to those that encode the nonstructural (NS) proteins NS3 and NS5 from flaviviruses were found in a salivary gland (SG) complementary DNA...
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StartPage 38
SubjectTerms Animals
Brazil
Cattle
Conserved Sequence - genetics
flavivirus
Flavivirus - chemistry
Flavivirus - classification
Flavivirus - isolation & purification
Gene Library
Hydrophobic and Hydrophilic Interactions
NS3
NS5
PARASITOLOGY
Phylogeny
Polymerase Chain Reaction
Rhipicephalus - virology
Rhipicephalus microplus
RNA Helicases - chemistry
RNA, Viral - isolation & purification
Sequence Alignment - statistics & numerical data
Sequence Analysis, Protein - methods
Serine Endopeptidases - chemistry
tick
Tissue Extracts - analysis
Transcriptome - genetics
TROPICAL MEDICINE
Viral Nonstructural Proteins - chemistry
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Title Characterisation of divergent flavivirus NS3 and NS5 protein sequences detected in Rhipicephalus microplus ticks from Brazil
URI http://www.bioline.org.br/abstract?id=oc14006
https://www.ncbi.nlm.nih.gov/pubmed/24626302
https://search.proquest.com/docview/1521912112
https://pubmed.ncbi.nlm.nih.gov/PMC4005522
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762014000100038&lng=en&tlng=en
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