In Vitro Susceptibility Characteristics of Cryptococcus neoformans Varieties from AIDS Patients in Goiânia, Brazil

• Published in: Memórias do Instituto Oswaldo Cruz Vol. 98; no. 6; pp. 839 - 841
• Main Authors: de F L Fernandes, Orionalda, Passos, Xisto S, Souza, Lúcia K H, Miranda, André T B, Cerqueira, Carlos Henrique P V, Silva, Maria do Rosário R
• Format: Journal Article
• Language: English
• Published: Brazil Fundação Oswaldo Cruz, Fiocruz 01-09-2003; Instituto Oswaldo Cruz, Ministério da Saúde; Fundação Oswaldo Cruz (FIOCRUZ)
• Subjects: Adolescent; Adult; Agglutination Tests; AIDS-Related Opportunistic Infections - microbiology; Amphotericin B - pharmacology; Antifungal Agents - pharmacology; antifungal susceptibility; Brazil; Cryptococcus neoformans; Cryptococcus neoformans - classification; Cryptococcus neoformans - drug effects; Cryptococcus neoformans - isolation & purification; Female; Fluconazole - pharmacology; Goiânia; Humans; Itraconazole - pharmacology; Male; Microbial Sensitivity Tests; Middle Aged; PARASITOLOGY; serotypes; serotypes - antifungal susceptibility - Cryptococcus neoformans - Goiânia - Brazil; Serotyping; TROPICAL MEDICINE; Brazil, Goiania; Brazil; Goiânia; serotypes; Cryptococcus neoformans; antifungal susceptibility
• ISSN: 1678-8060; 0074-0276; 0074-0276; 1678-8060
• DOI: 10.1590/S0074-02762003000600022

Sixty clinical isolates of Cryptococcus neoformans from AIDS from
Goiânia, state of Goiás, Brazil, were characterized according
to varieties, serotypes and tested for antifungal susceptibility. To
differentiate the two varieties was used
L-canavanine-glycine-bromothymol blue medium and to separate the
serotypes was used slide agglutination test with Crypto Check Iatron.
The Minimal Inhibitory Concentration (MIC) of fluconazole,
itraconazole, and amphotericin B were determined by the National
Committee for Clinical Laboratory Standards macrodilution method. Our
results identified 56 isolates as C. neoformans var. neoformans
serotype A and 4 isolates as C. neoformans var. gattii serotype B. MIC
values for C. neoformans var. gattii were higher than C. neoformans
var. neoformans. We verified that none isolate was resistant to
itraconazole and to amphotericin B, but one C. neoformans var.
neoformans and three C. neoformans var. gattii isolates were resistant
to fluconazole. The presence of C. neoformans var. gattii fluconazole
resistant indicates the importance of determining not only the variety
of C. neoformans infecting the patients but also measuring the MIC of
the isolate in order to properly orient treatment.