Taeniosis-cysticercosis complex in individuals of a peasants' settlement (Teodoro Sampaio, Pontal of Paranapanema, SP, Brazil)

• Published in: Memórias do Instituto Oswaldo Cruz Vol. 101; no. 1; pp. 15 - 20
• Main Authors: Prestes-Carneiro, Luiz Euribel, Freitas, Selma de Bastos Zambeli de, Zago, Sueli Cristina Schadeck, Miguel, Nadia Araújo, Primo, Oswaldo Bill, Iha, Alberto Hiroshi, Espíndola, Noeli Maria, Vaz, Adelaide José
• Format: Journal Article
• Language: English
• Published: Brazil Fundação Oswaldo Cruz, Fiocruz 01-02-2006; Instituto Oswaldo Cruz, Ministério da Saúde; Fundação Oswaldo Cruz (FIOCRUZ)
• Subjects: Adolescent; Adult; Aged; Animals; Antibodies, Helminth - blood; Biomarkers - blood; Brazil; Brazil - epidemiology; Child; Cysticercosis - diagnosis; Cysticercosis - epidemiology; enzyme linked immunoabsorbent assay; Enzyme-Linked Immunosorbent Assay; Feces - parasitology; Female; Humans; IgE; immunoblot; Immunoblotting; Immunoglobulin E - blood; Luminescent Measurements; Male; Middle Aged; PARASITOLOGY; Taenia solium cysticercus antibodies; Taenia solium cysticercus antibodies - enzyme linked immunoabsorbent assay - immunoblot - IgE - Brazil; Taeniasis - diagnosis; Taeniasis - epidemiology; TROPICAL MEDICINE; Brazil; Brazil; Taenia solium cysticercus antibodies; enzyme linked immunoabsorbent assay; IgE; immunoblot
• ISSN: 1678-8060; 0074-0276; 0074-0276; 1678-8060
• DOI: 10.1590/S0074-02762006000100004

In order to evaluate the taeniosis-cysticercosis complex in a
population of a peasants' settlement, located at Teodoro Sampaio, state
of São Paulo, Brazil (longitude 52°36'12", latitude
22°17'12") a series of laboratory markers were determined. After
signing an informed consent, participants answered a standardized
questionnaire. To determine anti-Taenia solium cysticercus antibodies,
the samples were tested by enzyme linked immunoabsorbent assay using
18-and 14-kDa antigen proteins from vesicular fluid of Taenia
crassiceps (VF-Tcra). The reactive and inconclusive ELISA samples were
tested by immunoblotting. Total IgE levels were determined by
chemmiluminescence's assay and hemogram by flow cytometer flux counter.
A total of 84 individuals, 5.9% presented anti-T. solium cysticercus
antibodies in ELISA and 3.6% were strongly reactive in the 18/14 kDa
immunoblotting confirmatory test. All of the individuals with positive
antibodies showed elevated Total IgE levels. We conclude that the
frequency of anti-T. solium cysticercus antibodies in this population
is higher than other regions considered endemic in São Paulo.
Thus, it is important to carry out surveys in Peasants' settlement
areas with the objective of establishing public health measures for
prevention and control of infectious diseases such as
taeniosis-cysticercosis.