PRESENCE OF DOUBLE-STRANDED RNAs IN HUMAN AND CALF ISOLATES OF CRYPTOSPORIDIUM PARVUM

• Published in: The Journal of parasitology Vol. 86; no. 2; pp. 275 - 282
• Main Authors: Khramtsov, Nikolai V, Chung, Peter A, Dykstra, Christine C, Griffiths, Jeffrey K, Morgan, Una M, Arrowood, Michael J, Upton, Steve J
• Format: Journal Article
• Language: English
• Published: Lawrence, KS American Society of Parasitologists 01-04-2000
• Subjects: Animals; Base Sequence; Biological and medical sciences; Blotting, Northern; calves; Cattle; Cattle Diseases - parasitology; complementary DNA; cryptosporidiosis; Cryptosporidiosis - parasitology; Cryptosporidium parvum; Cryptosporidium parvum - classification; Cryptosporidium parvum - genetics; Cryptosporidium parvum - isolation & purification; Double stranded RNA; Feces - parasitology; Fundamental and applied biological sciences. Psychology; genbank/af169922; genbank/af169923; genbank/af169924; genbank/af169925; genbank/af169926; genbank/af169927; genbank/af169928; genbank/af169929; genbank/af169930; genbank/af169931; genbank/af169932; genbank/af169933; genbank/af169934; genbank/af169935; genbank/af169936; genbank/af169937; genbank/af169938; genbank/af169939; genbank/af169940; genbank/af169941; genbank/af169942; genbank/af169943; genbank/af169944; genbank/af169945; genetic variation; Genetics of eukaryotes. Biological and molecular evolution; GENETICS-EVOLUTION; Genotype; Genotypes; Humans; Molecular Sequence Data; Nucleic acids; nucleotide sequences; Nucleotides; Oocysts; Parasite hosts; Parasitology; Phylogeny; Polymorphism, Restriction Fragment Length; Population genetics, reproduction patterns; Protozoa; Reverse Transcriptase Polymerase Chain Reaction; RNA; RNA, Double-Stranded - chemistry; RNA, Double-Stranded - isolation & purification; RNA, Protozoan - chemistry; RNA, Protozoan - isolation & purification; Sequence Alignment; Viruses; Human; Protozoa; Host parasite relation; Apicomplexa; Protozoal disease; Double stranded RNA; Bovine; Nucleotide sequence; Parasite; Genotype; Parasitosis; Population genetics; Young animal; Infection; Cryptosporidiosis; Vertebrata; Mammalia; Restriction fragment length polymorphism; Cryptosporidium parvum; Isolate; Artiodactyla; Intraspecific comparison; Ungulata
• ISSN: 0022-3395; 1937-2345
• DOI: 10.1645/0022-3395(2000)086[0275:PODSRI]2.0.CO;2

We examined the occurrence of 2 virus-like double-stranded (ds)RNAs in human and calf isolates of Cryptosporidium parvum senso latu and other microorganisms, including 7 other members of the genus. A total of 32 isolates of C. parvum, 16 from humans (5 from acquired immune deficiency syndrome patients) and 16 from calves, were analyzed. Ethidium bromide staining, or Northern blot analysis, or reverse transcription/polymerase chain reaction, or all 3 methods, revealed that both genotype 1 and genotype 2 isolates of C. parvum possessed these dsRNAs. No other Cryptosporidium spp. or other organisms examined possessed these dsRNAs. Comparison analysis of partial cDNA sequences of dsRNAs from human and calf isolates revealed a high degree of similarity (>92% and >93% identical nucleotides for large and small dsRNAs, respectively). Slight, consistent differences in nucleotide sequences could be seen at select sites and were associated with an isolate being either genotype 1 or 2. Because of the widespread distribution of the dsRNAs, the similarity of these molecules between isolates, and high host specificity, these nucleic acids may prove to represent species-specific molecular markers for C. parvum. Evidence also suggests that the dsRNA can be utilized for molecular genotyping of C. parvum.