Thiosemicarbazone-Pt(II) Complex Causes a Growth Inhibitory Effect on Human Mesenchymal Stem Cells

• Published in: Medicinal chemistry (Shp-sariqah, United Arab Emirates) Vol. 11; no. 7; p. 670
• Main Authors: Garcia-Ruiz, Josefa Predestinacion, Matesanz Garcia, Ana Isabel, Souza, Ana Perez, Castelo, Pilar Souza
• Format: Journal Article
• Language: English
• Published: Netherlands 01-01-2015
• Subjects: Cell Proliferation - drug effects; Cytoskeleton - drug effects; Cytoskeleton - metabolism; Humans; Immunomodulation - drug effects; Mesenchymal Stromal Cells - cytology; Mesenchymal Stromal Cells - drug effects; Models, Molecular; Molecular Conformation; Neoplasm Metastasis; Neovascularization, Pathologic; Organometallic Compounds - chemistry; Organometallic Compounds - pharmacology; Platinum - chemistry; Thiosemicarbazones - chemistry
• DOI: 10.2174/1573406411666150514101026
• EISSN: 1875-6638

We showed di[3,5-diacetyl-1,2,4-triazolbis(4-cyclohexylthiosemicarbazonato) platinum(II)] complex, (W8), endowed with important antitumor properties. Here, we analysed whether W8 can affect human bone marrow-derived Mesenchymal Stem Cells, (hMSCs), involved in tissue repair, immunomodulatory properties and also capacity for homing to injure-tumor sites in ovarian cancer. Specifically, we analysed the effect of W8 on cell proliferation, response to scratch, and whether copper-derived cellular mechanism is used by this platinum(II) complex being studied. Results showed that W8 causes a significant inhibition of cell proliferation at µM concentration. This effect is directly related to the alteration of cytoskeletal proteins and inhibition of the response to scratch induced by the presence of foetal bovine serum. This strongly supports the notion of W8 triggers the energetic metabolism of hMSCs and adds an extra support by the results showing W8 relationship with the cellular copper ions. W8, acting in hMSCs, regulates in addition the inhibition of cell proliferation, the inhibition of tumor angiogenesis and metastasis.