Quantitative analysis of changes in endometrial gland morphology during the bovine oestrous cycle and their association with progesterone levels

• Published in: Reproduction (Cambridge, England) Vol. 134; no. 2; pp. 365 - 371
• Main Authors: Wang, C K, Robinson, R S, Flint, A P F, Mann, G E
• Format: Journal Article
• Language: English
• Published: England Society for Reproduction and Fertility 01-08-2007; BioScientifica Ltd
• Subjects: Animals; Cattle - anatomy & histology; Cattle - blood; Decidua - anatomy & histology; Estrous Cycle - physiology; Female; Image Processing, Computer-Assisted; Microscopy; Microtomy; Progesterone - blood; Staining and Labeling
• ISSN: 1470-1626; 1741-7899
• DOI: 10.1530/REP-06-0133

This study describes a digital technique for uterine morphometry and its application to endometrial structure during the bovine oestrous cycle. Neither the number nor the size of uterine gland ducts changed during the cycle but a reduction in total endometrial area from days 0 to 8 after oestrus led to an increase in the proportion of the endometrium occupied by gland ducts (gland duct density). This effect on day 8 was maintained to day 16. When endometrial morphology was related to circulating progesterone concentrations on days 5 and 8 of the luteal phase, no relationships were found on day 5, but on day 8, a high progesterone concentration was associated with an increased number of gland ducts. Furthermore, in animals slaughtered on day 8, a high progesterone concentration on day 5 was associated with decreased gland duct size, though a simultaneous decrease in endometrial area led to an increase in gland duct density. The results suggest that contrary to expectation, endometrial glands do not grow and regress during the oestrous cycle, although cyclic changes in endometrial area controlled by progesterone lead to changes in gland duct density.