FIVE CASES OF DIPHYLLOBOTHRIUM NIHONKAIENSE INFECTION WITH DISCOVERY OF PLEROCERCOIDS FROM AN INFECTIVE SOURCE, ONCORHYNCHUS MASOU ISHIKAWAE

• Published in: The Journal of parasitology Vol. 87; no. 1; pp. 96 - 100
• Main Authors: Ando, Katsuhiko, Ishikura, Ken, Nakakugi, Tetsuya, Shimono, Yoshiki, Tamai, Takuya, Sugawa, Masahiro, Limviroj, Wutipong, Chinzei, Yasuo
• Format: Journal Article
• Language: English
• Published: Lawrence, KS American Society of Parasitologists 01-02-2001
• Subjects: Adolescent; Animals; Base Sequence; Biological and medical sciences; Biological taxonomies; Diphyllobothriasis - parasitology; Diphyllobothrium - anatomy & histology; Diphyllobothrium - classification; Diphyllobothrium - genetics; Diphyllobothrium - growth & development; Diseases caused by cestodes; DNA, Ribosomal Spacer - genetics; Eggs; Electron Transport Complex IV - genetics; Female; Food Parasitology; Freshwater fishes; Gene banks; Genes, Helminth; Helminthic diseases; Humans; Infections; Infectious diseases; LIFE CYCLES-SURVEY; Male; Medical sciences; Middle Aged; Miscellaneous; Mitochondrial DNA; Molecular Sequence Data; Nucleotide sequences; Oncorhynchus - classification; Oncorhynchus - genetics; Oncorhynchus - parasitology; Parasitic diseases; Parasitology; Plerocercoids; Sequence Analysis, DNA; Worms; Asia; Japan; Host parasite relation; Edible fish; Oncorhynchus masou; Cytochrome-c oxidase; Uncooked food; Mitochondrial DNA; Cestoda; Subunit; Pisces; Salmonidae; Human; Nucleotide sequence; Enzyme; Plathelmintha; Parasite; Host; Parasitosis; Helminthiasis; Cestode disease; Infection; Case study; Vertebrata; Diet; Morphology; Helmintha; Specific identification; Oxidoreductases; Invertebrata
• ISSN: 0022-3395; 1937-2345
• DOI: 10.1645/0022-3395(2001)087[0096:FCODNI]2.0.CO;2

Five persons from 2 families residing at Miyama Town, Mie Prefecture, Japan, ingested fresh raw fish Oncorhynchus sp. on 9 May 1999 that was caught at Owase district in Mie. They all expelled diphyllobothriid cestodes 11–37 days after ingesting the fish. The parasites were morphologically identical to Diphyllobothrium nihonkaienseYamane et al., 1986. Five plerocercoids were detected from a portion of the fish. Nucleotide sequence of a region of the cytochrome c oxidase subunit I gene of mitochondrial DNA from an adult worm was identical with that from the plerocercoid. The fish was identified as Oncorhynchus masou ishikawae according to the nucleotide sequence of the nuclear ribosomal second internal transcribed spacer region II gene. This is the first record of D. nihonkaiense plerocercoids from O. m. ishikawae.