Formic acid decalcification of bone marrow trephines degrades DNA: alternative use of EDTA allows the amplification and sequencing of relatively long PCR products

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Bibliographic Details
Published in:	Molecular pathology Vol. 53; no. 6; p. 336
Main Authors:	Sarsfield, P, Wickham, C L, Joyner, M V, Ellard, S, Jones, D B, Wilkins, B S
Format:	Journal Article
Language:	English
Published:	England BMJ Publishing Group Ltd and Association of Clinical Pathologists 01-12-2000 British Medical Journal Publishing Group
Subjects:	Bone Marrow - pathology Correspondence Decalcification Technique DNA - isolation & purification Edetic Acid - pharmacology Formates - pharmacology Humans
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Author	Wilkins, B S Wickham, C L Sarsfield, P Ellard, S Jones, D B Joyner, M V
AuthorAffiliation	2 Department of Haematology, Royal Devon and Exeter NHS Healthcare Trust 4 Department of Pathology, Level E , South Block, Southampton General Hospital, Tremona Road, Southampton SO16 6YD, UK 3 Molecular Genetics, Division of Clinical Science, School of Postgraduate Medicine and Health Sciences, University of Exeter, Exeter EX2 5AX, UK 1 Department of Pathology, Royal Devon and Exeter NHS Healthcare Trust, Barrack Road, Exeter EX2 5DW, UK
AuthorAffiliation_xml	– name: 2 Department of Haematology, Royal Devon and Exeter NHS Healthcare Trust – name: 4 Department of Pathology, Level E , South Block, Southampton General Hospital, Tremona Road, Southampton SO16 6YD, UK – name: 1 Department of Pathology, Royal Devon and Exeter NHS Healthcare Trust, Barrack Road, Exeter EX2 5DW, UK – name: 3 Molecular Genetics, Division of Clinical Science, School of Postgraduate Medicine and Health Sciences, University of Exeter, Exeter EX2 5AX, UK
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SubjectTerms	Bone Marrow - pathology Correspondence Decalcification Technique DNA - isolation & purification Edetic Acid - pharmacology Formates - pharmacology Humans
Title	Formic acid decalcification of bone marrow trephines degrades DNA: alternative use of EDTA allows the amplification and sequencing of relatively long PCR products
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