Digital dermatitis in beef cattle
All samples for PCR analysis were transported on ice and later stored at -20°C. Bacterial isolation of tissues and DNA extraction from treponeme cultures was performed as described previously ( Evans and others 2008 ). For PCR analysis, tissues from DD lesions were thawed and DNA extracted using a D...
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| Published in: | Veterinary record Vol. 173; no. 23; p. 582 |
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| Abstract | All samples for PCR analysis were transported on ice and later stored at -20°C. Bacterial isolation of tissues and DNA extraction from treponeme cultures was performed as described previously ( Evans and others 2008 ). For PCR analysis, tissues from DD lesions were thawed and DNA extracted using a Dneasy kit (Qiagen, UK) according to the manufacturer's instructions, and genomic DNA stored at -20°C. Samples were subjected to nested PCR specific for the three BDD treponeme groups described by Evans and others (2008 , 2009) with resulting fragments encompassing 300-500 bp of the 16S rRNA gene. Sample number Group-specific PCR* Treponema genus-specific PCR Details Lesion site 1 2 3 1 Gloucestershire Farm 1, animal 1 Hind right foot + + - + 2 Gloucestershire Farm 1, animal 2 Front left foot + + + + 3 Gloucestershire Farm 2, animal 1 Hind right foot + + + + 4 Gloucestershire Farm 2, animal 2 Hind right foot - + + + *Groups 1, 2 and 3 are Treponema medium/T vincentii-like, Treponema phagedenis-like and Treponema pedis spirochaetes, respectively, which are routinely found in BDD lesions Spirochaetes were successfully isolated from lesion sample 1 and 16S rRNA gene sequencing were carried out as previously described ( Evans and others 2008 ). Preventive Veterinary Medicine 104, 44-52 Stamm L.V. Bergen H. L. Walker R. L. ( 2002 ) Molecular typing of papillomatous digital dermatitis-associated Treponema isolates based on analysis of 16S-23S ribosomal DNA intergenic spacer regions. |
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| AbstractList | All samples for PCR analysis were transported on ice and later stored at -20°C. Bacterial isolation of tissues and DNA extraction from treponeme cultures was performed as described previously ( Evans and others 2008 ). For PCR analysis, tissues from DD lesions were thawed and DNA extracted using a Dneasy kit (Qiagen, UK) according to the manufacturer's instructions, and genomic DNA stored at -20°C. Samples were subjected to nested PCR specific for the three BDD treponeme groups described by Evans and others (2008 , 2009) with resulting fragments encompassing 300-500 bp of the 16S rRNA gene. Sample number Group-specific PCR* Treponema genus-specific PCR Details Lesion site 1 2 3 1 Gloucestershire Farm 1, animal 1 Hind right foot + + - + 2 Gloucestershire Farm 1, animal 2 Front left foot + + + + 3 Gloucestershire Farm 2, animal 1 Hind right foot + + + + 4 Gloucestershire Farm 2, animal 2 Hind right foot - + + + *Groups 1, 2 and 3 are Treponema medium/T vincentii-like, Treponema phagedenis-like and Treponema pedis spirochaetes, respectively, which are routinely found in BDD lesions Spirochaetes were successfully isolated from lesion sample 1 and 16S rRNA gene sequencing were carried out as previously described ( Evans and others 2008 ). Preventive Veterinary Medicine 104, 44-52 Stamm L.V. Bergen H. L. Walker R. L. ( 2002 ) Molecular typing of papillomatous digital dermatitis-associated Treponema isolates based on analysis of 16S-23S ribosomal DNA intergenic spacer regions. |
| Author | Blowey, R. Carter, S. D. Sullivan, L. E. Duncan, J. S. Grove-White, D. Evans, N. J. |
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| Cites_doi | 10.3168/jds.2008-1135 10.1128/JCM.01914-08 10.1016/j.prevetmed.2011.10.002 10.2460/ajvr.2000.61.928 10.1136/vr.c5487 10.3168/jds.2008-1590 10.1016/j.vetmic.2007.12.019 10.1128/JCM.40.9.3463-3469.2002 10.1136/vr.140.24.620 10.1128/JCM.00670-08 10.1016/j.vetmic.2005.10.016 |
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| Copyright | British Veterinary Association British Veterinary Association 2013 Copyright: 2014 British Veterinary Association |
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Milan, September 9 to 13, 1974 year: 1974 ident: e_1_2_3_4_1 contributor: fullname: Cheli R. – ident: e_1_2_3_7_1 doi: 10.1136/vr.140.24.620 – ident: e_1_2_3_12_1 doi: 10.1128/JCM.00670-08 – ident: e_1_2_3_13_1 doi: 10.1016/j.vetmic.2005.10.016 |
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| SubjectTerms | Animals Beef Cattle Cattle Diseases - diagnosis Cattle Diseases - microbiology Deoxyribonucleic acid Dermatitis Digital Dermatitis - diagnosis Digital Dermatitis - microbiology Disease DNA Foot conditions Infectious diseases Lameness Male PCR Ribosomal DNA Spirochaetes United Kingdom Veterinary medicine |
| Title | Digital dermatitis in beef cattle |
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