Digital dermatitis in beef cattle

• Published in: Veterinary record Vol. 173; no. 23; p. 582
• Main Authors: Sullivan, L. E., Carter, S. D., Blowey, R., Duncan, J. S., Grove-White, D., Evans, N. J.
• Format: Journal Article
• Language: English
• Published: England BMJ Publishing Group Limited 14-12-2013; Blackwell Publishing Ltd
• Subjects: Animals; Beef; Cattle; Cattle Diseases - diagnosis; Cattle Diseases - microbiology; Deoxyribonucleic acid; Dermatitis; Digital Dermatitis - diagnosis; Digital Dermatitis - microbiology; Disease; DNA; Foot conditions; Infectious diseases; Lameness; Male; PCR; Ribosomal DNA; Spirochaetes; United Kingdom; Veterinary medicine; United Kingdom > UK; United States > US; Foot conditions; Spirochaetes; Infectious diseases; Lameness; Cattle; PCR
• ISSN: 0042-4900; 2042-7670
• DOI: 10.1136/vr.101802

All samples for PCR analysis were transported on ice and later stored at -20°C. Bacterial isolation of tissues and DNA extraction from treponeme cultures was performed as described previously ( Evans and others 2008 ). For PCR analysis, tissues from DD lesions were thawed and DNA extracted using a Dneasy kit (Qiagen, UK) according to the ­manufacturer's instructions, and genomic DNA stored at -20°C. Samples were subjected to nested PCR specific for the three BDD treponeme groups described by Evans and others (2008 , 2009) with resulting fragments encompassing 300-500 bp of the 16S rRNA gene. Sample number Group-specific PCR* Treponema genus-specific PCR Details Lesion site 1 2 3 1 Gloucestershire Farm 1, animal 1 Hind right foot + + - + 2 Gloucestershire Farm 1, animal 2 Front left foot + + + + 3 Gloucestershire Farm 2, animal 1 Hind right foot + + + + 4 Gloucestershire Farm 2, animal 2 Hind right foot - + + + *Groups 1, 2 and 3 are Treponema medium/T vincentii-like, Treponema phagedenis-like and Treponema pedis spirochaetes, respectively, which are routinely found in BDD lesions Spirochaetes were successfully isolated from lesion sample 1 and 16S rRNA gene sequencing were carried out as previously described ( Evans and others 2008 ). Preventive Veterinary Medicine 104, 44-52 Stamm L.V. Bergen H. L. Walker R. L. ( 2002 ) Molecular typing of papillomatous digital dermatitis-associated Treponema isolates based on analysis of 16S-23S ribosomal DNA intergenic spacer regions.