Possible cause of outbreak of prolific vomiting in dogs

• Published in: Veterinary record Vol. 186; no. 10; p. 324
• Main Authors: Smith, Shirley L, Singleton, David A, Noble, PJ, Radford, Alan D, Brant, Beth, Pinchbeck, Gina L, Appleton, Charlotte, Jewell, Chris, Newton, Richard, Cuartero, Carmen Tamayo, Sánchez-Vizcaíno, Fernando
• Format: Journal Article
• Language: English
• Published: England BMJ Publishing Group Limited 14-03-2020; Blackwell Publishing Ltd
• Subjects: Coronaviruses; Laboratories; Vomiting
• ISSN: 0042-4900; 2042-7670
• DOI: 10.1136/vr.m972

Data collected by practices participating in the Small Animal Veterinary Surveillance Network (SAVSNET) also showed a profound rise in dogs presenting for non-specific gastroenteric disease, probably starting around November 2019 (see Fig 1 of the original letter). Here, we describe the results of testing samples submitted by owners and vets from suspected prolific vomiting cases and 16 non-affected controls for CECoV. Nucleic acid was extracted using the QiaAMP (Qiagen) viral RNA extraction kit, reverse transcribed using random hexamers and superscript III and tested for CECoV by PCR of the M gene.1 Testing PCR-positive for CECoV was significantly associated with prolific vomiting cases (16 of 39 cases; 41 per cent) (P=0.005; Fisher’s exact test); none of the 16 control cases tested positive for CECoV.