ISOLATION AND GENOTYPING OF CLOSTRIDIUM PERFRINGENS FROM FREE-LIVING SOUTH AMERICAN COATI (NASUA NASUA)

The importance of Clostridium perfringens for most wild animal species remains unclear. This study aimed to isolate and genotype C. perfringens in stool samples from free-living South American coati (Nasua nasua) in Brazil. Forty-six free-living N. nasua were trapped and stool samples were collected...

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Published in:	Journal of zoo and wildlife medicine Vol. 47; no. 1; pp. 333 - 336
Main Authors:	Silva, Rodrigo O. S, Almeida, Lara R, Junior, Carlos A. Oliveira, Lima, Paula C. S, Soares, Danielle F. M, Pereira, Pedro L. L, Silva, Israel J, Lobato, Francisco C. F
Format:	Journal Article
Language:	English
Published:	United States American Association of Zoo Veterinarians 01-03-2016
Subjects:	Animals Animals, Wild Brief Communication BRIEF COMMUNICATIONS Clostridium Infections - microbiology Clostridium Infections - veterinary Clostridium perfringens - genetics Clostridium perfringens - isolation & purification coatis diarrhea enteritis Female Genotype Male Nasua nasua procyonid Procyonidae β-2 Brazil coatis enteritis procyonid β-2 Nasua nasua diarrhea
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Abstract	The importance of Clostridium perfringens for most wild animal species remains unclear. This study aimed to isolate and genotype C. perfringens in stool samples from free-living South American coati (Nasua nasua) in Brazil. Forty-six free-living N. nasua were trapped and stool samples were collected. Two different protocols for C. perfringens isolation were tested: direct plating onto selective agar and pre-enrichment in broth followed by plating in selective agar. Clostridium perfringens type A was isolated from 15 (32.6%) animals by direct plating and 36 (78.3%) animals by broth PE, and the rate of isolation was significantly different between these two methods (P < 0.01). Twelve of the 36 (33.3%) isolated strains by the PE protocol were positive for the β-2 toxin-encoding gene (cpb2) whereas the enterotoxin-encoding gene (cpe) and necrotic enteritis like-B toxin gene (netb) were not found. These results suggest that C. perfringens is commonly part of the microbiota of free-living coatis. Additionally, the use of a PE protocol appears to be essential for studies on C. perfringens in this species.
AbstractList	The importance of Clostridium perfringens for most wild animal species remains unclear. This study aimed to isolate and genotype C. perfringens in stool samples from free-living South American coati (Nasua nasua) in Brazil. Forty-six free-living N. nasua were trapped and stool samples were collected. Two different protocols for C. perfringens isolation were tested: direct plating onto selective agar and pre-enrichment in broth followed by plating in selective agar. Clostridium perfringens type A was isolated from 15 (32.6%) animals by direct plating and 36 (78.3%) animals by broth PE, and the rate of isolation was significantly different between these two methods (P < 0.01). Twelve of the 36 (33.3%) isolated strains by the PE protocol were positive for the β-2 toxin-encoding gene (cpb2) whereas the enterotoxin-encoding gene (cpe) and necrotic enteritis like-B toxin gene (netb) were not found. These results suggest that C. perfringens is commonly part of the microbiota of free-living coatis. Additionally, the use of a PE protocol appears to be essential for studies on C. perfringens in this species. The importance of Clostridium perfringens for most wild animal species remains unclear. This study aimed to isolate and genotype C. perfringens in stool samples from free-living South American coati (Nasua nasua) in Brazil. Forty-six free-living N. nasua were trapped and stool samples were collected. Two different protocols for C. perfringens isolation were tested: direct plating onto selective agar and pre-enrichment in broth followed by plating in selective agar. Clostridium perfringens type A was isolated from 15 (32.6%) animals by direct plating and 36 (78.3%) animals by broth PE, and the rate of isolation was significantly different between these two methods (P < 0.01). Twelve of the 36 (33.3%) isolated strains by the PE protocol were positive for the β-2 toxin-encoding gene (cpb2) whereas the enterotoxin-encoding gene (cpe) and necrotic enteritis like-B toxin gene (netb) were not found. These results suggest that C. perfringens is commonly part of the microbiota of free-living coatis. Additionally, the use of a PE protocol appears to be essential for studies on C. perfringens in this species.
Author	Lobato, Francisco C. F Silva, Rodrigo O. S Pereira, Pedro L. L Silva, Israel J Almeida, Lara R Lima, Paula C. S Junior, Carlos A. Oliveira Soares, Danielle F. M
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Cites_doi	10.20506/rst.29.3.2010 10.1016/j.cvsm.2010.12.005 10.1128/JCM.37.2.358-361.1999 10.1590/S0102-09352006000500043 10.2217/fmb.13.168 10.1016/j.anaerobe.2014.06.012 10.1590/1808-1657v75p5132008 10.1089/vbz.2010.0096 10.1638/2011-0265R.1 10.1590/S0103-84782011005000100 10.1590/S1517-83822013005000008 10.1016/j.cvex.2011.05.002 10.1016/j.jcpa.2007.04.018 10.7589/2013-01-005 10.1177/104063870501700216 10.1111/j.1863-2378.2011.01412.x 10.1016/j.vetmic.2012.04.012 10.1111/evj.12046 10.2460/javma.241.6.712 10.3390/toxins2071913 10.1016/j.anaerobe.2014.09.012 10.1371/journal.ppat.0040026 10.1177/104063871102300232 10.1111/j.1439-0450.2004.00802.x 10.1638/2010-0197.1
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SubjectTerms	Animals Animals, Wild Brief Communication BRIEF COMMUNICATIONS Clostridium Infections - microbiology Clostridium Infections - veterinary Clostridium perfringens - genetics Clostridium perfringens - isolation & purification coatis diarrhea enteritis Female Genotype Male Nasua nasua procyonid Procyonidae β-2
Title	ISOLATION AND GENOTYPING OF CLOSTRIDIUM PERFRINGENS FROM FREE-LIVING SOUTH AMERICAN COATI (NASUA NASUA)
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