MICROPROPAGATION OF LEPIDIUM VIRGINICUM (BRASSICACEAE), A PLANT WITH ANTIPROTOZOAL ACTIVITY

• Published in: In vitro cellular & developmental biology. Plant Vol. 42; no. 6; pp. 596 - 600
• Main Authors: OSUNA, LIDIA, TAPIA-PÉREZ, MARÍA ESTHER, FIGUEROA, ODETTE, JIMÉNEZ-FERRER, ENRIQUE, GARDUÑO-RAMÍREZ, MARÍA LUISA, GONZÁLEZ-GARZA, MARÍA TERESA, CARRANZA-ROSALES, PILAR, CRUZ-VEGA, DELIA ELVA
• Format: Journal Article
• Language: English
• Published: Heidelberg Tissue Culture Association 01-11-2006; CABI Publishing; Springer Nature B.V
• Subjects: acclimatization; Acetic acid; antiprotozoal activity; Antiprotozoals; benzyladenine; Cotyledons; cytokinins; Developing countries; explants; Flowers & plants; Growth regulators; Hypocotyls; indole acetic acid; indole butyric acid; Inhibitory concentration 50; kinetin; LDCs; Lepidium virginicum; Liver diseases; Medical treatment; Medicinal plants; methanol; MICROPROPAGATION; morphogenesis; Mortality; Plant growth; Plant roots; Plants; Protozoa; roots; Seeds; shoots; Trophozoites; wild plants
• ISSN: 1054-5476; 1475-2689
• DOI: 10.1079/IVP2006826

Micropropagation is a technique to ensure a constant and uniform source of medicinal plants. In this report, we describe the micropropagation of Lepidium virginicum L. (Brassicaceae), a wild plant used as an antiamoebic in traditional Mexican medicine. In vitro-germinated seeds were cultured in Murashige and Skoog (MS) medium to obtain pathogen-free cotyledons, hypocotyls, and apical bud (AB) explants. For induction of morphogenesis, the effect of cytokinins, benzyladenine (BA) and kinetin (KN), combined with auxin, indole-3-acetic acid (IAA) was evaluated. The best rate of shoot proliferation was induced 15 d after culture on MS mineral medium supplemented with IAA:KN (0.57:13.94 μM) from AB explants. Maximum shoot elongation was achieved without plant growth regulators. The effect of indole-3-butyric acid (IBA) (14.76 μM) was evaluated for in vitro root induction; 60 d after culture all the shoots had developed roots. All rooted plants were successfully transferred to pots and 100% acclimatized in ex vitro conditions. The methanol extracts from the micropropagated active explants of L. virginicum showed and IC50 antiprotozoal value between 141.90 and 268.53 μg ml−1.