MicroRNA Expression Profile in Early-Stage Breast Cancers

• Published in: MicroRNA (Shariqah, United Arab Emirates) Vol. 13; no. 1; p. 71
• Main Authors: Patel, Krishna, Rao, Deva Magendhra, Sundersingh, Shirley, Velusami, Sridevi, Rajkumar, Thangarajan, Nair, Bipin, Pandey, Akhilesh, Chatterjee, Aditi, Mani, Samson, Gowda, Harsha
• Format: Journal Article
• Language: English
• Published: United Arab Emirates 01-01-2024
• Subjects: Biomarkers, Tumor - genetics; Breast Neoplasms - genetics; Breast Neoplasms - mortality; Breast Neoplasms - pathology; Carcinoma, Ductal, Breast - genetics; Carcinoma, Ductal, Breast - pathology; Carcinoma, Intraductal, Noninfiltrating - genetics; Carcinoma, Intraductal, Noninfiltrating - pathology; Female; Gene Expression Profiling; Gene Expression Regulation, Neoplastic - genetics; Humans; MicroRNAs - genetics; Middle Aged; Neoplasm Staging; Transcriptome - genetics; ceRNA; breast cancer; benign tumor; Next Generation sequencing; DCIS; small RNAs
• ISSN: 2211-5374
• DOI: 10.2174/0122115366256479231003064842

Breast cancer is one of the leading causes of cancer deaths in women. Early diagnosis offers the best hope for a cure. Ductal carcinoma in situ is considered a precursor of invasive ductal carcinoma of the breast. In this study, we carried out microRNA sequencing from 7 ductal carcinoma in situ (DCIS), 6 infiltrating ductal carcinomas (IDC Stage IIA) with paired normal, and 5 unpaired normal breast tissue samples.
We have deployed miRge for microRNA analysis, DESeq for differential expression analysis, and Cytoscape for competing endogenous RNA network investigation.
Here, we identified 76 miRNAs that were differentially expressed in DCIS and IDC. Additionally, we provide preliminary evidence of miR-365b-3p and miR-7-1-3p being overexpressed, and miR-6507-5p, miR-487b-3p, and miR-654-3p being downregulated in DCIS relative to normal breast tissue. We also identified a miRNA miR-766-3p that was overexpressed in earlystage IDCs. The overexpression of miR-301a-3p in DCIS and IDC was confirmed in 32 independent breast cancer tissue samples.
Higher expression of miR-301a-3p is associated with poor overall survival in The Cancer Genome Atlas Breast Cancer (TCGA-BRCA) dataset, indicating that it may be associated with DCIS at high risk of progressing to IDC and warrants deeper investigation.