ANTI-BACTERIAL AND ANTI-OXIDANT ACTIVITIES OF LEAF EXTRACTS OF COMBRETUM VENDAE (COMBRETECACEA) AND THE ISOLATION OF AN ANTI-BACTERIAL COMPOUND

• Published in: African journal of traditional, complementary, and alternative medicines Vol. 11; no. 5; pp. 73 - 77
• Main Authors: Komape, Nancy Patience Motlalepula, Aderogba, Mutalib, Bagla, Victor Patrick, Masoko, Peter, Eloff, Jacobus Nicolaas
• Format: Journal Article
• Language: English
• Published: Nigeria African Ethnomedicines Network 01-01-2014
• Subjects: acacetin; Anti-Bacterial Agents - isolation & purification; Anti-Bacterial Agents - pharmacology; Anti-oxidant; Antioxidants - isolation & purification; Antioxidants - pharmacology; Bacteria - drug effects; Bio-autography; Biphenyl Compounds - metabolism; Combretum - chemistry; Combretum vendae; Enterococcus faecalis - drug effects; Flavones - isolation & purification; Flavones - pharmacology; Medicine, African Traditional; Microbial Sensitivity Tests; Minimum Inhibitory Concentration; Phytochemical analysis; Picrates - metabolism; Plant Extracts - chemistry; Plant Extracts - pharmacology; Plant Leaves - chemistry; Pseudomonas aeruginosa - drug effects; South Africa; Staphylococcus aureus - drug effects; South Africa; Combretum vendae; Minimum Inhibitory Concentration; Phytochemical analysis; acacetin; Bio-autography; Anti-oxidant
• ISSN: 0189-6016; 2505-0044
• DOI: 10.4314/ajtcam.v11i5.12

Background: Combretum vendae A.E. van Wyk (Combretaceae) is used for
the treatment of bacterial related infections and oxidative related
diseases by indigenous people of South Africa. Dried leaves extracts of
C. vendae were investigated for bioactivity against a variety of
bacterial strains and their antioxidant potential evaluated. Materials
and methods: Constituents of leaf material were serially extracted
using solvents of varying polarities, TLC chromatograms of the
fractions were sprayed with 2,2 diphenyl-1-picrylhydrazyl (DPPH) to
determine the presence of antioxidant compounds. Bio-autography was
used to determine the number of antibacterial compounds active against
Staphylococcus aureus , Enterococcus faecalis , Eschericha coli and
Pseudomonas aeruginosa . Minimum inhibitory concentration (MIC) values
were determined using serial microplate dilution method. The chloroform
fraction was subjected to bio-assay guided column chromatography to
isolate the active compound. Results: The mass extracted by different
solvents was below 10% dry weight. MIC values for different extracts
against different pathogens ranges from 0.08 to 0.64 mg/ml. The
compound isolated was identified as acacetin having an Rf value of 0.28
following elution in the Ethanol: Methanol: Water [E: M: W (10: 1.35: 1
v/v). Acacetin had MIC values ranging from 0.16 to 0.35 mg/ml.
Conclusion: We report for the first time the isolation of acacetin as
the main antibacterial compound from the leaves of Combretum vendae.