In Vitro Development of Bovine Nuclear Transfer Embryos from Transgenic Clonal Lines of Adult and Fetal Fibroblast Cells of the Same Genotype1
This study examined bovine cloning strategies that may be used for gene targeting in animals of known phenotypic traits. Fibroblast cells derived from an adult and a fetus of the same genotype were transfected with a plasmid (pEGFP-N1) containing the enhanced green fluorescence protein and neomycin-...
Saved in:
| Published in: | Biology of reproduction Vol. 66; no. 6; pp. 1768 - 1774 |
|---|---|
| Main Authors: | , , , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
01-06-2002
|
| Subjects: | |
| Online Access: | Get full text |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| Abstract | This study examined bovine cloning strategies that may be used for gene targeting in animals of known phenotypic traits. Fibroblast cells derived from an adult and a fetus of the same genotype were transfected with a plasmid (pEGFP-N1) containing the enhanced green fluorescence protein and neomycin-resistant genes. After transfecting 2 × 105 cells, 49 adult and 35 fetal cell colonies were obtained. Green fluorescence expression was observed in 35 out of 49 (71.4%) adult clones and in 30 out of 35 (85.7%) fetal clones. Developmental rates to the blastocyst stage following nuclear transfer (NT) did not differ among nontransfected cell lines (adult, 20.0%; NT fetal, 18.3%), whereas developmental rates were significantly lower for adult and fetal cell lines expressing enhanced green fluorescent protein (EGFP; 11.3% and 6.4%, respectively, P < 0.05). However, there was no decrease in NT developmental rates (19.8%) when donor nuclei from EGFP-transfected cell lines not expressing EGFP but retaining neomycin-resistant gene expression were used as donor nuclei. NT embryos from adult and fetal cell lines had similar morphology, cell number, and ploidy. The results indicated that adult and NT fetal cells (identical genotype) can complete clonal propagation, including transfection and selection, and can be used to produce transgenic NT embryos; however, a possible deleterious effect of EGFP on embryo development should be considered in future gene targeting studies. |
|---|---|
| AbstractList | This study examined bovine cloning strategies that may be used for gene targeting in animals of known phenotypic traits. Fibroblast cells derived from an adult and a fetus of the same genotype were transfected with a plasmid (pEGFP-N1) containing the enhanced green fluorescence protein and neomycin-resistant genes. After transfecting 2 × 105 cells, 49 adult and 35 fetal cell colonies were obtained. Green fluorescence expression was observed in 35 out of 49 (71.4%) adult clones and in 30 out of 35 (85.7%) fetal clones. Developmental rates to the blastocyst stage following nuclear transfer (NT) did not differ among nontransfected cell lines (adult, 20.0%; NT fetal, 18.3%), whereas developmental rates were significantly lower for adult and fetal cell lines expressing enhanced green fluorescent protein (EGFP; 11.3% and 6.4%, respectively, P < 0.05). However, there was no decrease in NT developmental rates (19.8%) when donor nuclei from EGFP-transfected cell lines not expressing EGFP but retaining neomycin-resistant gene expression were used as donor nuclei. NT embryos from adult and fetal cell lines had similar morphology, cell number, and ploidy. The results indicated that adult and NT fetal cells (identical genotype) can complete clonal propagation, including transfection and selection, and can be used to produce transgenic NT embryos; however, a possible deleterious effect of EGFP on embryo development should be considered in future gene targeting studies. |
| Author | Stice, Steven L Tumlin, Monica Arat, Sezen Gibbons, John Jacek Rzucidlo, S Respess, Donald S |
| Author_xml | – sequence: 1 givenname: Sezen surname: Arat fullname: Arat, Sezen organization: Research Institute for Genetic Engineering and Biotechnology, TUBITAK, Gebze, Kocaeli 41470, Turkey – sequence: 2 givenname: John surname: Gibbons fullname: Gibbons, John organization: Prolinia Inc., Athens, Georgia 30602 – sequence: 3 givenname: S surname: Jacek Rzucidlo fullname: Jacek Rzucidlo, S organization: Prolinia Inc., Athens, Georgia 30602 – sequence: 4 givenname: Donald S surname: Respess fullname: Respess, Donald S organization: Prolinia Inc., Athens, Georgia 30602 – sequence: 5 givenname: Monica surname: Tumlin fullname: Tumlin, Monica organization: Prolinia Inc., Athens, Georgia 30602 – sequence: 6 givenname: Steven L surname: Stice fullname: Stice, Steven L email: sstice@arches.uga.edu organization: Prolinia Inc., Athens, Georgia 30602 |
| BookMark | eNqNkUFOwzAQRS1UJNrCCdj4Ail27DjJspS2VKpgQWEb2fEYghw7stNKvQRnJqUsWLIa6f95s3gzQSPnHSB0S8mMkjK7U423AbrgtRAzMaO5KC7QmGZpmeSpKEZoTAgRCWOCXaFJjJ-EUM5SNkZfG4ffmj54_AAHsL5rwfXYG3zvD40D_LSvLciAd0G6aCDgZavC0Udsgm_P6Tu4psYL6520eDtA8cTP9d72WDqNV9APxapRwSsrY48XYO3PTv8B-EW2gNfgfH_sgF6jSyNthJvfOUWvq-Vu8Zhsn9ebxXybqJSkRSJ4XppaGqYyIyhhuSw5g8JoRnhK1RClynBaZERQnoEutMi41towKQRXkk0RO9-tg48xgKm60LQyHCtKqpPS6q_SSlQnpQNFz9RQDg_4F_MNe2-A9Q |
| CitedBy_id | crossref_primary_10_1371_journal_pone_0077798 crossref_primary_10_1016_j_theriogenology_2005_09_031 crossref_primary_10_1016_j_theriogenology_2019_06_004 crossref_primary_10_1016_j_pnsc_2008_07_023 crossref_primary_10_1095_biolreprod_105_045138 crossref_primary_10_1371_journal_pone_0062457 crossref_primary_10_1007_s11248_020_00207_w crossref_primary_10_1016_S0093_691X_02_01294_3 crossref_primary_10_1111_j_1432_0436_2004_07201003_x crossref_primary_10_1002_mrd_20071 crossref_primary_10_1016_j_theriogenology_2004_02_006 crossref_primary_10_1007_s11248_012_9634_y crossref_primary_10_1016_j_theriogenology_2009_11_008 crossref_primary_10_1530_rep_1_00089 crossref_primary_10_1017_S0967199415000519 crossref_primary_10_1111_j_1469_7998_2008_00490_x crossref_primary_10_1111_j_1439_0531_2006_00723_x crossref_primary_10_1292_jvms_66_1567 crossref_primary_10_1002_mrd_20558 crossref_primary_10_1155_2014_281031 crossref_primary_10_4142_jvs_2014_15_2_225 crossref_primary_10_1002_mrd_20283 crossref_primary_10_1017_S0967199410000651 crossref_primary_10_1002_mrd_20640 crossref_primary_10_1007_s11248_014_9818_8 crossref_primary_10_1016_j_anireprosci_2011_12_007 crossref_primary_10_1016_j_chemosphere_2009_08_043 crossref_primary_10_1089_cell_2017_0013 crossref_primary_10_17485_ijst_2018_v11i25_128251 crossref_primary_10_1007_s10238_010_0112_7 crossref_primary_10_1089_clo_2005_7_238 crossref_primary_10_1007_s10561_012_9292_6 crossref_primary_10_1095_biolreprod_107_066662 crossref_primary_10_1262_jrd_10_063A crossref_primary_10_1095_biolreprod_104_027193 crossref_primary_10_1095_biolreprod_109_081083 crossref_primary_10_1002_mrd_20372 crossref_primary_10_1071_RD11026 crossref_primary_10_1002_mrd_20297 crossref_primary_10_1095_biolreprod_104_031591 crossref_primary_10_1007_s11248_008_9189_0 crossref_primary_10_4142_jvs_2016_17_2_145 crossref_primary_10_1016_j_anireprosci_2006_12_012 crossref_primary_10_1016_j_theriogenology_2004_09_056 crossref_primary_10_1016_j_livsci_2008_06_029 crossref_primary_10_1111_rda_12140 crossref_primary_10_1262_jrd_20102 crossref_primary_10_1016_j_theriogenology_2010_04_017 crossref_primary_10_1002_mrd_10362 crossref_primary_10_1016_j_theriogenology_2017_10_010 crossref_primary_10_1292_jvms_66_257 crossref_primary_10_1016_j_theriogenology_2008_04_007 crossref_primary_10_1016_j_theriogenology_2012_05_027 crossref_primary_10_1016_j_theriogenology_2005_10_014 crossref_primary_10_1515_pjvs_2016_0029 crossref_primary_10_1007_s11248_011_9503_0 crossref_primary_10_1002_mrd_20740 crossref_primary_10_1530_REP_08_0469 crossref_primary_10_1017_S0967199407004236 crossref_primary_10_1017_S0967199406003716 crossref_primary_10_1071_RD08143 crossref_primary_10_1016_j_biochi_2012_08_009 crossref_primary_10_1095_biolreprod_102_010066 crossref_primary_10_1089_cell_2010_0022 |
| Cites_doi | 10.1095/biolreprod60.6.1273 10.1095/biolreprod60.4.996 10.1016/0014-5793(95)01162-8 10.1126/science.278.5346.2130 10.1126/science.289.5482.1188 10.1071/RD00021 10.1016/S0093-691X(99)00149-1 10.1038/8632 10.1016/S0168-9525(00)89090-3 10.1002/(SICI)1098-2795(199909)54:1<43::AID-MRD6>3.0.CO;2-N 10.1007/BF01868659 10.1126/science.282.5396.2095 10.1002/(SICI)1098-2795(199904)52:4<406::AID-MRD9>3.0.CO;2-P 10.1016/S0378-4320(01)00106-3 10.1095/biolreprod64.5.1487 10.1095/biolreprod64.3.849 10.1002/(SICI)1098-2795(200006)56:2<145::AID-MRD4>3.0.CO;2-8 10.1095/biolreprod54.1.53 10.1038/35024082 10.1016/S0015-0282(16)57739-5 10.5713/ajas.2000.856 10.1002/(SICI)1098-2795(199803)49:3<298::AID-MRD10>3.0.CO;2-T 10.1126/science.280.5367.1256 10.1073/pnas.97.3.990 10.1093/carcin/21.3.477 10.1006/dbio.1995.1018 10.1002/mrd.1043 10.1016/S0925-4773(98)00093-8 10.1095/biolreprod62.5.1135 10.1002/mrd.1057 10.1038/35016604 10.1038/385810a0 10.1089/hum.1997.8.11-1313 10.1016/S0093-691X(00)00444-1 10.1139/g80-067 10.1126/science.8303295 10.1126/science.288.5466.665 10.1126/science.277.5327.831 10.1002/(SICI)1098-2795(199911)54:3<264::AID-MRD7>3.0.CO;2-Y 10.1002/1098-2795(200101)58:1<15::AID-MRD3>3.0.CO;2-Y 10.1095/biolreprod64.6.1695 10.1095/biolreprod62.6.1640 |
| ContentType | Journal Article |
| Copyright | Society for the Study of Reproduction |
| Copyright_xml | – notice: Society for the Study of Reproduction |
| DBID | AAYXX CITATION |
| DOI | 10.1095/biolreprod66.6.1768 |
| DatabaseName | CrossRef |
| DatabaseTitle | CrossRef |
| DatabaseTitleList | |
| DeliveryMethod | fulltext_linktorsrc |
| Discipline | Anatomy & Physiology Biology |
| EISSN | 1529-7268 |
| EndPage | 1774 |
| ExternalDocumentID | 10_1095_biolreprod66_6_1768 |
| GroupedDBID | - 08R 0R 186 1TH 23N 2WC 3O- 3V. 48X 53G 5GY 5RE 5VS 5WD 7X7 88E 8FI 8FJ AABJS AABMN AACFU AAIMJ AAJQQ AAPBV AAPPN AAPQZ AAPSS AAUQX ABFLS ABPTD ABSAR ABSGY ABUWG ACFRR ACGFS ACNCT ACUFI ACUTJ ADBBV ADEIU ADGZP ADHKW ADIPN ADOYD ADRTK ADVEK AEDJY AELNO AELWJ AEMDU AENEX AENZO AETBJ AEWNT AFFNX AFFZL AFKRA AFULF AFXEN AGINJ AHMBA AIKOY AKPMI ALMA_UNASSIGNED_HOLDINGS APIBT ARIXL ASAOO ATDFG AYOIW AZQFJ BAWUL BAYMD BBAFP BBNVY BCRHZ BENPR BEYMZ BHONS BHPHI BPHCQ BSWAC BVXVI BYORX C1A C45 CAG CDBKE COF CS3 DAKXR DC7 DIK DPPUQ DU5 E3Z EBS EF EJD F5P FA8 FHSFR FYUFA GAUVT GJ GJXCC H13 HCIFZ H~9 IAO IHR INH INIJC JH KM KOP KQ8 KSN M1P M7P MBTAY MVM NLBLG O9- OBOKY ODMLO OHT OK1 OVD OWPYF P2P PAFKI PEELM PQ0 PQEST PQQKQ PQUKI PRINS PROAC PSQYO Q5J RBO RHF ROL ROX ROZ TCN TEORI TLC TOX TR2 TSR VH1 WH7 WOQ X YAYTL YKOAZ YXANX ZGI ZXP --- -JH -~X .GJ 0R~ 6J9 AAPXW AARHZ AAUAY AAVAP AAYXX ABEJV ABJNI ABMNT ABXVV ACGFO ADHSS ADNWM ADQBN AEPYG AFFIJ AFGWE AFNWH AFOFC AI. AJEEA ALIPV ATGXG CITATION F9R FLUFQ FOEOM JXSIZ KBUDW KSI NOMLY OJZSN RUSNO W8F WHG YHG ZCN ~EF ~KM |
| ID | FETCH-LOGICAL-b2028-6479fcaf3b5f61037a943e8fd30421b6102bf418506145ed8d654dddf3a664ba3 |
| ISSN | 0006-3363 |
| IngestDate | Thu Nov 21 21:46:20 EST 2024 Thu Nov 04 13:51:22 EDT 2021 |
| IsPeerReviewed | true |
| IsScholarly | true |
| Issue | 6 |
| Language | English |
| LinkModel | OpenURL |
| MergedId | FETCHMERGED-LOGICAL-b2028-6479fcaf3b5f61037a943e8fd30421b6102bf418506145ed8d654dddf3a664ba3 |
| PageCount | 7 |
| ParticipantIDs | crossref_primary_10_1095_biolreprod66_6_1768 bioone_primary_10_1095_biolreprod66_6_1768 |
| PublicationCentury | 2000 |
| PublicationDate | 20020600 2002-06-01 |
| PublicationDateYYYYMMDD | 2002-06-01 |
| PublicationDate_xml | – month: 06 year: 2002 text: 20020600 |
| PublicationDecade | 2000 |
| PublicationTitle | Biology of reproduction |
| PublicationYear | 2002 |
| References | ( key 20170224202927_R27) 2000; 407 ( key 20170224202927_R42) 1995; 167 ( key 20170224202927_R2) 1998; 280 ( key 20170224202927_R41) 1977; 41 ( key 20170224202927_R10) 2000; 97 ( key 20170224202927_R18) 1997; 8 ( key 20170224202927_R17) 2000; 12 ( key 20170224202927_R43) 1980; 22 ( key 20170224202927_R14) 2001; 64 ( key 20170224202927_R44) 1999; 60 ( key 20170224202927_R45) 2001; 59 ( key 20170224202927_R9) 1999; 52 ( key 20170224202927_R23) 1998; 49 ( key 20170224202927_R19) 2001; 64 ( key 20170224202927_R35) 1998; 76 ( key 20170224202927_R6) 1997; 385 ( key 20170224202927_R38) 2001; 64 ( key 20170224202927_R33) 1999; 54 ( key 20170224202927_R1) 2000; 13 ( key 20170224202927_R26) 2000; 289 ( key 20170224202927_R29) 2000; 405 ( key 20170224202927_R40) 1995; 64 ( key 20170224202927_R16) 2001; 58 ( key 20170224202927_R24) 2001; 55 ( key 20170224202927_R20) 1997; 277 ( key 20170224202927_R21) 2001; 67 ( key 20170224202927_R4) 1997; 278 ( key 20170224202927_R31) 2000; 288 ( key 20170224202927_R39) 1990 ( key 20170224202927_R8) 2000; 62 ( key 20170224202927_R12) 1994; 263 ( key 20170224202927_R36) 1995; 375 ( key 20170224202927_R46) 1996; 54 ( key 20170224202927_R37) 1999; 54 ( key 20170224202927_R3) 1999; 17 ( key 20170224202927_R30) 2001; 64 ( key 20170224202927_R7) 1999; 60 ( key 20170224202927_R15) 2000; 62 ( key 20170224202927_R28) 1998; 282 ( key 20170224202927_R11) 2001; 60 ( key 20170224202927_R22) 1982; 67 ( key 20170224202927_R25) 2001; 55 ( key 20170224202927_R32) 2000; 21 ( key 20170224202927_R13) 1995; 11 ( key 20170224202927_R5) 2000; 56 ( key 20170224202927_R34) 1999; 52 |
| References_xml | – volume: 60 start-page: 1273 year: 1999 ident: key 20170224202927_R44 article-title: A high proportion of bovine blastocysts produced in vitro are mixoploid publication-title: Biol Reprod doi: 10.1095/biolreprod60.6.1273 – volume: 60 start-page: 996 year: 1999 ident: key 20170224202927_R7 article-title: Production of cloned calves following nuclear transfer with cultured adult mural granulosa cells publication-title: Biol Reprod doi: 10.1095/biolreprod60.4.996 – volume: 41 start-page: 47 year: 1977 ident: key 20170224202927_R41 article-title: Development of cytochalasin B-induced tetraploid and diploid/tetraploid mosaic mouse embryos publication-title: J Embryol Exp Morphol – volume: 375 start-page: 125 year: 1995 ident: key 20170224202927_R36 article-title: A rapid and non-invasive selection of transgenic embryos before implantation using green fluorescent protein (GFP) publication-title: FEBS Lett doi: 10.1016/0014-5793(95)01162-8 – volume: 278 start-page: 2130 year: 1997 ident: key 20170224202927_R4 article-title: Human factor IX transgenic sheep production by transfer of nuclei from transfected fatal fibroblasts publication-title: Science doi: 10.1126/science.278.5346.2130 – volume: 289 start-page: 1188 year: 2000 ident: key 20170224202927_R26 article-title: Pig cloning by microinjection of fetal fibroblast nuclei publication-title: Science doi: 10.1126/science.289.5482.1188 – volume: 64 start-page: 173 year: 2001 ident: key 20170224202927_R19 article-title: Bovine cloning using adult donor cells treated with roscovitine publication-title: Biol Reprod – volume: 12 start-page: 1 year: 2000 ident: key 20170224202927_R17 article-title: In vitro development of green fluorescent protein (GFP) transgenic bovine embryos after nuclear transfer using different cell cycles and passages of fetal fibroblasts publication-title: Reprod Fertil Dev doi: 10.1071/RD00021 – volume: 52 start-page: 527 year: 1999 ident: key 20170224202927_R9 article-title: Production of calves by transfer of nuclei from cultured somatic cells obtained from Japanese black bulls publication-title: Theriogenology doi: 10.1016/S0093-691X(99)00149-1 – volume: 17 start-page: 456 year: 1999 ident: key 20170224202927_R3 article-title: Production of goats by somatic cell nuclear transfer publication-title: Nat Biotechnol doi: 10.1038/8632 – volume: 11 start-page: 320 year: 1995 ident: key 20170224202927_R13 article-title: Using GFP to see the light publication-title: Trends Genet doi: 10.1016/S0168-9525(00)89090-3 – volume: 54 start-page: 43 year: 1999 ident: key 20170224202927_R37 article-title: Efficient selection of transgenic mouse embryos using EGFP as a marker gene publication-title: Mol Reprod Dev doi: 10.1002/(SICI)1098-2795(199909)54:1<43::AID-MRD6>3.0.CO;2-N – volume: 67 start-page: 165 year: 1982 ident: key 20170224202927_R22 article-title: Electric field-induced cell-to-cell fusion publication-title: J Membr Biol doi: 10.1007/BF01868659 – volume: 282 start-page: 2095 year: 1998 ident: key 20170224202927_R28 article-title: Eight calves cloned from somatic cells of single adult publication-title: Science doi: 10.1126/science.282.5396.2095 – volume: 52 start-page: 406 year: 1999 ident: key 20170224202927_R34 article-title: Timing of DNA Integration, transgenic mosaicism, and pronuclear microinjection publication-title: Mol Reprod Dev doi: 10.1002/(SICI)1098-2795(199904)52:4<406::AID-MRD9>3.0.CO;2-P – volume: 67 start-page: 17 year: 2001 ident: key 20170224202927_R21 article-title: Bovine nuclear transfer embryos development using cells derived from a cloned fetus publication-title: Anim Reprod Sci doi: 10.1016/S0378-4320(01)00106-3 – volume: 64: start-page: 1487 year: 2001 ident: key 20170224202927_R30 article-title: Effect of fibroblast donor cell age and cell cycle on development of bovine nuclear transfer embryos in vitro publication-title: Biol Reprod doi: 10.1095/biolreprod64.5.1487 – volume: 64 start-page: 849 year: 2001 ident: key 20170224202927_R14 article-title: Generation of dwarf goat (Capra hircus) clones following nuclear transfer with transfected and nontransfected fetal fibroblasts and in vitro-matured oocytes publication-title: Biol Reprod doi: 10.1095/biolreprod64.3.849 – volume: 56 start-page: 145 year: 2000 ident: key 20170224202927_R5 article-title: Production of transgenic porcine blastocysts by nuclear transfer publication-title: Mol Reprod Dev doi: 10.1002/(SICI)1098-2795(200006)56:2<145::AID-MRD4>3.0.CO;2-8 – volume: 54 start-page: 53 year: 1996 ident: key 20170224202927_R46 article-title: Chromosomal abnormalities in bovine embryos and their influence on development publication-title: Biol Reprod doi: 10.1095/biolreprod54.1.53 – volume: 407 start-page: 86 year: 2000 ident: key 20170224202927_R27 article-title: Cloned pigs produced by nuclear transfer from adult somatic cells publication-title: Nature doi: 10.1038/35024082 – volume: 64 start-page: 382 year: 1995 ident: key 20170224202927_R40 article-title: Embryo morphology, developmental rates, and maternal age are correlated with chromosome abnormalities publication-title: Fertil Steril doi: 10.1016/S0015-0282(16)57739-5 – volume: 13 start-page: 856 year: 2000 ident: key 20170224202927_R1 article-title: Improvements in nuclear transfer procedures will increase commercial utilization of animal cloning publication-title: Asian-Australas J Anim Sci doi: 10.5713/ajas.2000.856 – volume: 49 start-page: 298 year: 1998 ident: key 20170224202927_R23 article-title: Parthenogenetic development and protein patterns of newly maturated bovine oocytes after chemical activation publication-title: Mol Reprod Dev doi: 10.1002/(SICI)1098-2795(199803)49:3<298::AID-MRD10>3.0.CO;2-T – volume: 280 start-page: 1256 year: 1998 ident: key 20170224202927_R2 article-title: Cloned transgenic calves produced from non-quiescent fetal fibroblasts publication-title: Science doi: 10.1126/science.280.5367.1256 – volume: 97 start-page: 990 year: 2000 ident: key 20170224202927_R10 article-title: Six cloned calves produced from adult fibroblast cells after long-term culture publication-title: Proc Natl Acad Sci U S A doi: 10.1073/pnas.97.3.990 – volume: 21 start-page: 477 year: 2000 ident: key 20170224202927_R32 article-title: The role of senescence and immortalization in carcinogenesis publication-title: Carcinogenesis doi: 10.1093/carcin/21.3.477 – volume: 167 start-page: 213 year: 1995 ident: key 20170224202927_R42 article-title: Restricted distribution of tetraploid cells in mouse tetraploid-diploid chimeras publication-title: Dev Biol doi: 10.1006/dbio.1995.1018 – start-page: 229 volume-title: Advances in Veterinary Science and Comparative Medicine year: 1990 ident: key 20170224202927_R39 – volume: 59 start-page: 371 year: 2001 ident: key 20170224202927_R45 article-title: Remodeling of donor nuclei, DNA-synthesis, and ploidy of bovine cumulus cell nuclear transfer embryos: Effect of activation protocol publication-title: Mol Reprod Dev doi: 10.1002/mrd.1043 – volume: 76 start-page: 79 year: 1998 ident: key 20170224202927_R35 article-title: Generating green fluorescent mice by germline transmission of green fluorescent ES cells publication-title: Mech Dev doi: 10.1016/S0925-4773(98)00093-8 – volume: 62 start-page: 1135 year: 2000 ident: key 20170224202927_R8 article-title: Development rates of male bovine nuclear transfer embryos derived from adult and fetal cells publication-title: Biol Reprod doi: 10.1095/biolreprod62.5.1135 – volume: 60 start-page: 20 year: 2001 ident: key 20170224202927_R11 article-title: Production of transgenic bovine embryos by transfer of transfected granulosa cells into enucleated oocytes publication-title: Mol Reprod Dev doi: 10.1002/mrd.1057 – volume: 405 start-page: 1066 year: 2000 ident: key 20170224202927_R29 article-title: Production of gene-targeted sheep by nuclear transfer from cultured somatic cells publication-title: Nature doi: 10.1038/35016604 – volume: 385 start-page: 810 year: 1997 ident: key 20170224202927_R6 article-title: Viable offspring derived from fetal and adult mammalian cells publication-title: Nature doi: 10.1038/385810a0 – volume: 8 start-page: 1313 year: 1997 ident: key 20170224202927_R18 article-title: Green fluorescent protein retroviral vectors:low titer and high recombination frequency suggest a selective disadvantage publication-title: Hum Gene Ther doi: 10.1089/hum.1997.8.11-1313 – volume: 55 start-page: 35 year: 2001 ident: key 20170224202927_R25 article-title: Cloning to reproduce desired genotypes publication-title: Theriogenology doi: 10.1016/S0093-691X(00)00444-1 – volume: 22 start-page: 615 year: 1980 ident: key 20170224202927_R43 article-title: Chromosomal analysis of 159 bovine embryos collected 12 to 18 days after estrus publication-title: Can J Genet Cytol doi: 10.1139/g80-067 – volume: 263: start-page: 802 year: 1994 ident: key 20170224202927_R12 article-title: Green fluorescent protein as a marker for gene expression publication-title: Science doi: 10.1126/science.8303295 – volume: 288 start-page: 665 year: 2000 ident: key 20170224202927_R31 article-title: Extension of cell life-span and telomere length in animals cloned from senescent somatic cells publication-title: Science doi: 10.1126/science.288.5466.665 – volume: 277 start-page: 831 year: 1997 ident: key 20170224202927_R20 article-title: Bypass of senescence after disruption of p21CIP1/WAF1 gene in normal diploid human fibroblast publication-title: Science doi: 10.1126/science.277.5327.831 – volume: 54 start-page: 264 year: 1999 ident: key 20170224202927_R33 article-title: Adult cloning in cattle: potential of nuclei from a permanent cell line and from primary cultures publication-title: Mol Reprod Dev doi: 10.1002/(SICI)1098-2795(199911)54:3<264::AID-MRD7>3.0.CO;2-Y – volume: 58 start-page: 15 year: 2001 ident: key 20170224202927_R16 article-title: Developmental potential and transgene expression of porcine nuclear transfer embryos using somatic cells publication-title: Mol Reprod Dev doi: 10.1002/1098-2795(200101)58:1<15::AID-MRD3>3.0.CO;2-Y – volume: 55 start-page: 287 year: 2001 ident: key 20170224202927_R24 article-title: Effects of fibroblast source and tissue-culture medium on success of bovine nuclear transfer with transfected cells publication-title: Theriogenology – volume: 64 start-page: 1695 year: 2001 ident: key 20170224202927_R38 article-title: Clonal lines of transgenic fibroblast cells derived from the same fetus result in different development when used for nuclear transfer in pig publication-title: Biol Reprod doi: 10.1095/biolreprod64.6.1695 – volume: 62 start-page: 1640 year: 2000 ident: key 20170224202927_R15 article-title: Establish of a porcine cell line from in vitro-produced blastocysts and transfer of the cells into enucleated oocytes publication-title: Biol Reprod doi: 10.1095/biolreprod62.6.1640 |
| SSID | ssj0014323 |
| Score | 1.7008164 |
| Snippet | This study examined bovine cloning strategies that may be used for gene targeting in animals of known phenotypic traits. Fibroblast cells derived from an adult... |
| SourceID | crossref bioone |
| SourceType | Aggregation Database Publisher |
| StartPage | 1768 |
| SubjectTerms | assisted reproductive technology Contents developmental biology embryo |
| Title | In Vitro Development of Bovine Nuclear Transfer Embryos from Transgenic Clonal Lines of Adult and Fetal Fibroblast Cells of the Same Genotype1 |
| URI | http://www.bioone.org/doi/abs/10.1095/biolreprod66.6.1768 |
| Volume | 66 |
| hasFullText | 1 |
| inHoldings | 1 |
| isFullTextHit | |
| isPrint | |
| link | http://sdu.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwtV1Lb9NAEF6lRUhcELQgyktzQBwILvVrHR9DSGgA9dAWxC3yemeliMau8ji0P4LfzMzuOjaPQ3vgYiWT9Sjx92kzOzv7jRCvMpOjKYwKSgxVkKBOA0VLoUCWRuskx9hYIe3js-zk--DDOBn3ek2Tsdb2X5EmG2HNJ2dvgfbWKRnoNWFOV0KdrjfCfVr1v83Xy7pbDsQR4XtOHWD_hPWLi6UTNTdc4bFQy6t65c6ZWCu5npf90YXNEX7hsngbrrJQh91qmCAfoJzQOrtWFHuv-yO8cBrMHMSeFQsrZl1zcjf8bc_Y6z3RQJbStEqznSqAIXHR5mLxuj2e9nGuVF2t_iwa_lSU-KN_er0p59rtHW1j2lNcXeLKLw447-0_ajIbUVuBtZ2tZRDHfgJEP0FHeZBFrhVPM4O7vi2eqd3pOMz8QPRvXUegv_42KM4kXFn2yv1-KQ_lId-8I-5ENIPZtfr083Z7Kokj36LPfcFGzipP3_3DCQU3ZK0r7MQ-nSDm_IG471cfMHS0eSh6WO2J_WFVrOvFFbwGWw9sUdoTdz1e--LntALLKehwCmoDjlPgOQUNp8BzCphT0HIKHKfAcorvt5wC4hRYTkHLKbCc4jHEKWBOwZZTj8TXyfh8dBz4Ph6Bijh5K5MsN2VhYpUayedSizyJcWA0p9JCRaZIGRZR4uxEinqgZZporU1cSJmoIn4sdit6fE8EhMVROTARIndtIGcqSxBTcp_jUYyYH4g37lHPLp1Yy8wVWaSzLi4zOWNcDsTbBo6bDH96G9_PxL2W0s_F7nq5wRdiZ6U3Ly2VfgGiQaHU |
| link.rule.ids | 315,782,786,27933,27934 |
| linkProvider | Flying Publisher |
| openUrl | ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=In+Vitro+Development+of+Bovine+Nuclear+Transfer+Embryos+from+Transgenic+Clonal+Lines+of+Adult+and+Fetal+Fibroblast+Cells+of+the+Same+Genotype1&rft.jtitle=Biology+of+reproduction&rft.au=Arat%2C+Sezen&rft.au=Gibbons%2C+John&rft.au=Jacek+Rzucidlo%2C+S&rft.au=Respess%2C+Donald+S&rft.date=2002-06-01&rft.issn=0006-3363&rft.eissn=1529-7268&rft.volume=66&rft.issue=6&rft.spage=1768&rft.epage=1774&rft_id=info:doi/10.1095%2Fbiolreprod66.6.1768 |
| thumbnail_l | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=0006-3363&client=summon |
| thumbnail_m | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=0006-3363&client=summon |
| thumbnail_s | http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=0006-3363&client=summon |