Myeloperoxidase Content is a Marker of Systemic Inflammation in a Chronic Condition: The Example Given by the Periodontal Disease in Rats

• Published in: Mediators of Inflammation Vol. 2009; no. 2009; pp. 529 - 535
• Main Authors: Queiroz-Junior, Celso Martins, Pacheco, Cinthia Mara da Fonseca, Fonseca, Allyson Henrique, Klein, André, Caliari, Marcelo Vidigal, de Francischi, Janetti Nogueira
• Format: Journal Article
• Language: English
• Published: Cairo, Egypt Hindawi Limiteds 01-01-2009; Hindawi Puplishing Corporation; Hindawi Publishing Corporation; Hindawi; John Wiley & Sons, Inc; Hindawi Limited
• Subjects: Animals; Anti-Inflammatory Agents - therapeutic use; Biological and medical sciences; Biomarkers - metabolism; Celecoxib; Diagnosis; Facial bones, jaws, teeth, parodontium: diseases, semeiology; General aspects; Inflammation; Inflammation - metabolism; Leukocyte Count; Male; Mediators; Medical sciences; Morphine - therapeutic use; Neutrophils - cytology; Neutrophils - drug effects; Otorhinolaryngology. Stomatology; Periodontal disease; Periodontal Diseases - drug therapy; Periodontal Diseases - metabolism; Periodontal Diseases - pathology; Peroxidase; Peroxidase - metabolism; Physiological aspects; Pyrazoles - therapeutic use; Rats; Sulfonamides - therapeutic use; Brazil; Rat; Enzyme; Stomatology; Rodentia; Biological marker; Inflammation; Vertebrata; Chronic; Periodontal disease; Mammalia; Peroxidases; Animal; Peroxidase; Oxidoreductases
• ISSN: 0962-9351; 1466-1861
• DOI: 10.1155/2009/760837

The study aimed to evaluate the suitability of myeloperoxidase (MPO) content as a local indicator of chronic inflammation, using the periodontal disease model. Anesthetized adult male Holtzman rats had their second left maxilar molar tied by a thread for 11 days and were then killed. Blood samples and photographic images from histopathological inflamed and noninflamed (contralateral) neighboring gingivomucosal specimens were collected for cell counts and MPO level analysis. Diseased animals were also treated with pharmacological tools such as the anti-inflammatory drug celecoxib or the opioid morphine. Increased blood neutrophils and local cell numbers characterized diseased animals. However, local MPO content was increased in inflamed and noninflamed tissues from diseased animals. Celecoxib and morphine reduced blood neutrophils and bilateral MPO content, but only celecoxib reduced local cell numbers in diseased animals. It is concluded that MPO content is a good indicator of a systemic rather than a local inflammation in a chronic inflammatory condition.