Biphasic Extracellular Proteolytic Enzyme Activity in Benthic Water and Sediment in the Northwestern Mediterranean Sea
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| Published in: | Applied and Environmental Microbiology Vol. 65; no. 4; pp. 1619 - 1626 |
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| Main Authors: | , , , , |
| Format: | Journal Article |
| Language: | English |
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01-04-1999
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| AbstractList | In this study, we used the fact that bacteria are able to cleave a fluorogenic substrate analog (L-leucine-7-amido-4-methylcoumarin) to determine the maximal ectoproteolytic activities (V sub(m)) and affinities (K sub(m)) of natural benthic microbial communities by the multiconcentration kinetic method. This investigation was performed during the winter and summer of 1997 with a set of 36 samples of near-bottom water and sediment collected from a coastal area and an offshore area in the western part of the Gulf of Lions. The existence of biphasic microbial ectoproteolysis was statistically confirmed for both the near-bottom water and the sediment, regardless of the spatial and seasonal conditions. Globally, 72.2% of the entire set of bacterial consortia collected at the water-sediment boundary layer showed biphasic microbial kinetics. A specific estimator of the biphasicity indicated that deep benthic bacterial consortia responded better with episodic nutrient supplies than shallower benthic bacterial consortia responded. Classifications Services AEM Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue Spotlights in the Current Issue AEM About AEM Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy AEM RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • journals@asmusa.org Print ISSN: 0099-2240 Online ISSN: 1098-5336 Copyright © 2014 by the American Society for Microbiology. For an alternate route to AEM .asm.org, visit: AEM In this study, we used the fact that bacteria are able to cleave a fluorogenic substrate analog ( l -leucine-7-amido-4-methylcoumarin) to determine the maximal ectoproteolytic activities ( V m ) and affinities ( K m ) of natural benthic microbial communities by the multiconcentration kinetic method. This investigation was performed during the winter and summer of 1997 with a set of 36 samples of near-bottom water and sediment collected from a coastal area and an offshore area in the western part of the Gulf of Lions. The existence of biphasic microbial ectoproteolysis was statistically confirmed for both the near-bottom water and the sediment, regardless of the spatial and seasonal conditions. Globally, 72.2% of the entire set of bacterial consortia collected at the water-sediment boundary layer showed biphasic microbial kinetics. A specific estimator of the biphasicity indicated that deep benthic bacterial consortia responded better with episodic nutrient supplies than shallower benthic bacterial consortia responded. In this study, we used the fact that bacteria are able to cleave a fluorogenic substrate analog (L-leucine-7-amido-4-methylcoumarin) to determine the maximal ectoproteolytic activities (Vm) and affinities (Km) of natural benthic microbial communities by the multiconcentration kinetic method. In this study, we used the fact that bacteria are able to cleave a fluorogenic substrate analog (L-leucine-7-amido-4-methylcoumarin) to determine the maximal ectoproteolytic activities (Vm) and affinities (Km) of natural benthic microbial communities by the multiconcentration kinetic method. This investigation was performed during the winter and summer of 1997 with a set of 36 samples of near-bottom water and sediment collected from a coastal area and an offshore area in the western part of the Gulf of Lions. The existence of biphasic microbial ectoproteolysis was statistically confirmed for both the near-bottom water and the sediment, regardless of the spatial and seasonal conditions. Globally, 72.2% of the entire set of bacterial consortia collected at the water-sediment boundary layer showed biphasic microbial kinetics. A specific estimator of the biphasicity indicated that deep benthic bacterial consortia responded better with episodic nutrient supplies than shallower benthic bacterial consortia responded. |
| Author | Jean Garcin Thalia Polychronaki Olivier Tholosan François Lamy Armand Bianchi |
| AuthorAffiliation | Microbiologie Marine, Centre National de la Recherche Scientifique-Institut National des Sciences de l’Univers, Université de la Méditerranée, F-13288 Marseille Cedex 9, France, 1 and Institute of Marine Biology of Crete, GR71003, Iraklion, Crete, Greece 2 |
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| Cites_doi | 10.1007/978-1-4612-3090-8_7 10.1016/0278-4343(90)90077-Y 10.1016/0003-2697(90)90048-E 10.3354/meps093277 10.1016/0198-0149(87)90024-0 10.4319/lo.1989.34.6.0973 10.4319/lo.1997.42.2.0217 10.1016/0003-2697(92)90356-C 10.3354/meps006213 10.3354/meps102115 10.2307/2532412 10.1042/bj1390715 10.1007/978-1-4757-0387-0_14 10.1016/S0967-0645(96)00107-5 10.3354/meps168273 10.4319/lo.1983.28.1.0185 10.3354/meps011299 10.1038/370549a0 10.1016/0076-6879(92)10003-V 10.4319/lo.1965.10.1.0022 10.1016/0031-0182(90)90048-C 10.3354/meps016155 10.1007/978-1-4612-3090-8_3 10.1038/341437a0 10.3354/meps104299 10.1016/0198-0149(79)90028-1 10.1128/aem.53.8.1748-1755.1987 10.4319/lo.1983.28.6.1104 10.1038/302520a0 10.1007/978-1-4757-0387-0_19 10.4319/lo.1983.28.1.0190 10.3354/ame009017 10.4319/lo.1980.25.5.0943 10.3354/meps059249 10.1016/S0304-4203(97)00003-0 10.1038/333067a0 10.1016/0769-2609(88)90011-7 10.3354/meps033237 10.1128/aem.54.3.784-790.1988 10.1038/357670a0 10.1016/0022-0981(85)90167-4 10.1007/978-1-4612-3090-8_5 |
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| Copyright | 1999 INIST-CNRS Copyright American Society for Microbiology Apr 1999 Copyright © 1999, American Society for Microbiology 1999 |
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| Keywords | Marine environment Peptidases Enzymatic activity Enzyme Hydrolases Kinetics Kinetic parameter Sediments Microbial community Benthic zone |
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| Notes | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 Corresponding author. Mailing address: Microbiologie Marine, Centre National de la Recherche Scientifique-Institut National des Sciences de l’Univers-EP 2032, Université de la Méditerranée, F-13288 Marseille Cedex 9, France. Phone: 33 4 91 82 90 49. Fax: 33 4 91 82 90 51. E-mail: a-bianchi@luminy.univ-mrs.fr. |
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| References_xml | – ident: e_1_3_2_7_2 doi: 10.1007/978-1-4612-3090-8_7 – ident: e_1_3_2_17_2 doi: 10.1016/0278-4343(90)90077-Y – ident: e_1_3_2_19_2 doi: 10.1016/0003-2697(90)90048-E – ident: e_1_3_2_27_2 doi: 10.3354/meps093277 – ident: e_1_3_2_47_2 doi: 10.1016/0198-0149(87)90024-0 – ident: e_1_3_2_37_2 doi: 10.4319/lo.1989.34.6.0973 – ident: e_1_3_2_11_2 doi: 10.4319/lo.1997.42.2.0217 – volume: 15 start-page: 303 year: 1998 ident: e_1_3_2_12_2 article-title: Ectoaminopeptidase specificity and regulation in Antarctic marine pelagic microbial communities. publication-title: Mar. Ecol. Prog. Ser. contributor: fullname: Christian J. R. – volume: 29 start-page: 1 year: 1967 ident: e_1_3_2_14_2 article-title: The statistical analysis of enzyme kinetic data. publication-title: Adv. Enzymol. contributor: fullname: Cleland W. W. – ident: e_1_3_2_28_2 doi: 10.1016/0003-2697(92)90356-C – ident: e_1_3_2_5_2 doi: 10.3354/meps006213 – ident: e_1_3_2_50_2 – volume: 29 start-page: 25 year: 1985 ident: e_1_3_2_18_2 article-title: Adsorption des acides aminés par les sédiments marins et répercussion sur l’activité bactérienne. publication-title: Rapp. Comm. Int. Mer Mediterr. contributor: fullname: Drogue N. – ident: e_1_3_2_35_2 doi: 10.3354/meps102115 – ident: e_1_3_2_42_2 doi: 10.2307/2532412 – ident: e_1_3_2_21_2 doi: 10.1042/bj1390715 – ident: e_1_3_2_4_2 doi: 10.1007/978-1-4757-0387-0_14 – ident: e_1_3_2_51_2 doi: 10.1016/S0967-0645(96)00107-5 – ident: e_1_3_2_52_2 doi: 10.3354/meps168273 – ident: e_1_3_2_33_2 doi: 10.4319/lo.1983.28.1.0185 – ident: e_1_3_2_25_2 doi: 10.3354/meps011299 – ident: e_1_3_2_30_2 doi: 10.1038/370549a0 – ident: e_1_3_2_29_2 doi: 10.1016/0076-6879(92)10003-V – ident: e_1_3_2_55_2 – ident: e_1_3_2_57_2 doi: 10.4319/lo.1965.10.1.0022 – volume: 34 start-page: 1 year: 1990 ident: e_1_3_2_40_2 article-title: Microorganisms in marine sediments: considerations concerning activity measurements. publication-title: Arch. Hydrobiol. Beith. Ergebn. Limnol. contributor: fullname: Meyer-Reil L. A. – ident: e_1_3_2_53_2 doi: 10.1016/0031-0182(90)90048-C – ident: e_1_3_2_43_2 doi: 10.3354/meps016155 – volume: 12 start-page: 64 year: 1979 ident: e_1_3_2_31_2 article-title: Applicability and limitations of the Michaelis-Menten equation in microbial ecology. publication-title: Ergeb. Limnol. contributor: fullname: Krambeck C. – ident: e_1_3_2_13_2 doi: 10.1007/978-1-4612-3090-8_3 – ident: e_1_3_2_23_2 doi: 10.1038/341437a0 – ident: e_1_3_2_9_2 doi: 10.3354/meps104299 – ident: e_1_3_2_38_2 – ident: e_1_3_2_44_2 doi: 10.1016/0198-0149(79)90028-1 – ident: e_1_3_2_39_2 doi: 10.1128/aem.53.8.1748-1755.1987 – volume: 10 start-page: 223 year: 1996 ident: e_1_3_2_36_2 article-title: Variability in ectohydrolytic enzyme activities of pelagic marine bacteria and its significance for substrate processing in the sea. publication-title: Mar. Ecol. Prog. Ser. contributor: fullname: Martinez J. – volume: 31 start-page: 353 year: 1988 ident: e_1_3_2_48_2 article-title: Measurement of enzymatic solubilization of P.O.M. in marine sediment by using dye release-techniques. publication-title: Arch. Hydrobiol. Beith. Ergebn. Limnol. contributor: fullname: Reichardt W. – ident: e_1_3_2_24_2 doi: 10.4319/lo.1983.28.6.1104 – ident: e_1_3_2_8_2 doi: 10.1038/302520a0 – ident: e_1_3_2_2_2 doi: 10.1007/978-1-4757-0387-0_19 – ident: e_1_3_2_49_2 doi: 10.4319/lo.1983.28.1.0190 – ident: e_1_3_2_16_2 doi: 10.3354/ame009017 – ident: e_1_3_2_46_2 doi: 10.4319/lo.1980.25.5.0943 – volume: 80 start-page: 324 year: 1961 ident: e_1_3_2_56_2 article-title: Statistical estimations in enzyme kinetics. publication-title: Biochemistry contributor: fullname: Wilkinson G. N. – ident: e_1_3_2_20_2 doi: 10.3354/meps059249 – ident: e_1_3_2_45_2 doi: 10.1016/S0304-4203(97)00003-0 – ident: e_1_3_2_34_2 doi: 10.1038/333067a0 – volume: 7 start-page: 399 year: 1984 ident: e_1_3_2_6_2 article-title: A multiple corer for taking virtually undisturbed samples from shelf, bathyal and abyssal sediments. publication-title: Oceanol. Acta contributor: fullname: Barnett P. R. O. – ident: e_1_3_2_54_2 doi: 10.1016/0769-2609(88)90011-7 – ident: e_1_3_2_22_2 doi: 10.3354/meps033237 – ident: e_1_3_2_10_2 – ident: e_1_3_2_32_2 – ident: e_1_3_2_26_2 doi: 10.1128/aem.54.3.784-790.1988 – ident: e_1_3_2_3_2 doi: 10.1038/357670a0 – volume: 15 start-page: 217 year: 1998 ident: e_1_3_2_15_2 article-title: Exoproteolytic activity in an Atlantic pond (France): estimates of in situ activity. publication-title: Mar. Ecol. Prog. Ser. contributor: fullname: Crottereau C. – ident: e_1_3_2_58_2 doi: 10.1016/0022-0981(85)90167-4 – ident: e_1_3_2_41_2 doi: 10.1007/978-1-4612-3090-8_5 |
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Reddit... In this study, we used the fact that bacteria are able to cleave a fluorogenic substrate analog (L-leucine-7-amido-4-methylcoumarin) to determine the maximal... In this study, we used the fact that bacteria are able to cleave a fluorogenic substrate analog ( l -leucine-7-amido-4-methylcoumarin) to determine the maximal... |
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| StartPage | 1619 |
| SubjectTerms | Animal, plant and microbial ecology Bacteria Biological and medical sciences Enzymes Fundamental and applied biological sciences. Psychology General Microbial Ecology Greece L-Leucine-7-amido-4 methylcoumarin Mediterranean Sea Mediterranean Sea, Lions Gulf Microbial ecology Microbiology Oceans Sediments Various environments (extraatmospheric space, air, water) Water |
| Title | Biphasic Extracellular Proteolytic Enzyme Activity in Benthic Water and Sediment in the Northwestern Mediterranean Sea |
| URI | http://aem.asm.org/content/65/4/1619.abstract https://www.ncbi.nlm.nih.gov/pubmed/10103259 https://www.proquest.com/docview/205940415 https://search.proquest.com/docview/17361208 https://search.proquest.com/docview/1859301601 https://search.proquest.com/docview/26870355 https://pubmed.ncbi.nlm.nih.gov/PMC91229 |
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