Development of the GENIPOL European Flounder (Platichthys flesus) Microarray and Determination of Temporal Transcriptional Responses to Cadmium at Low Dose

We have constructed a high density, 13 270-clone cDNA array for the sentinel fish species European flounder (Platichthys flesus), combining clones from suppressive subtractive hybridization and a liver cDNA library; DNA sequences of 5211 clones were determined. Fish were treated by single intraperit...

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Bibliographic Details
Published in:	Environmental science & technology Vol. 40; no. 20; pp. 6479 - 6488
Main Authors:	Williams, Tim D, Diab, Amer M, George, Stephen G, Godfrey, Rita E, Sabine, Victoria, Conesa, Ana, Minchin, Steven D, Watts, Phil C, Chipman, James K
Format:	Journal Article
Language:	English
Published:	Washington, DC American Chemical Society 15-10-2006
Subjects:	Agnatha. Pisces Animal, plant and microbial ecology Animals Applied ecology Arrays Biological and medical sciences Cadmium Cadmium Chloride - administration & dosage Cadmium Chloride - pharmacology Cell cycle Cloning Deoxyribonucleic acid DNA Dose-Response Relationship, Drug Ecotoxicology, biological effects of pollution Environmental Monitoring - methods Flounder Flounder - genetics Flounder - growth & development Fundamental and applied biological sciences. Psychology Gene expression Gene Expression Profiling Gene Expression Regulation, Developmental - drug effects General aspects Genomics Liver Liver - drug effects Liver - growth & development Liver - metabolism Metals Oligonucleotide Array Sequence Analysis - methods Platichthys flesus Pollutants Reverse Transcriptase Polymerase Chain Reaction Transcription, Genetic - drug effects Vertebrates: general zoology, morphology, phylogeny, systematics, cytogenetics, geographical distribution Europe Freshwater environment Marine environment Aquatic environment Cadmium Vertebrata Pisces Low dose Platichthys flesus Heavy metal
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