High-Fat-Diet-Induced Oxidative Stress Linked to the Increased Colonization of Lactobacillus sakei in an Obese Population

• Published in: Microbiology spectrum Vol. 9; no. 1; p. e0007421
• Main Authors: Lee, Jee-Yon, Bae, Eunsoo, Kim, Hwa Young, Lee, Kang-Mu, Yoon, Sang Sun, Lee, Duk-Chul
• Format: Journal Article
• Language: English
• Published: United States American Society for Microbiology 03-09-2021
• Subjects: Animals; Clinical Microbiology; Colon - metabolism; Colon - microbiology; Diet, High-Fat - adverse effects; Feces - microbiology; Female; Gastrointestinal Microbiome; gut microbiome; high-fat diet; Humans; inflammation; Lactobacillus - classification; Lactobacillus - genetics; Lactobacillus - growth & development; Lactobacillus - isolation & purification; Lactobacillus sakei; Lactobacillus sakei - genetics; Lactobacillus sakei - growth & development; Lactobacillus sakei - isolation & purification; Male; Mice; Mice, Inbred C57BL; obesity; Obesity - metabolism; Obesity - microbiology; Oxidative Stress; Reactive Oxygen Species - metabolism; reactive oxygen stress; Research Article; gut microbiome; reactive oxygen stress; inflammation; Lactobacillus sakei; obesity; high-fat diet
• ISSN: 2165-0497; 2165-0497
• DOI: 10.1128/Spectrum.00074-21

Obesity is a major public health problem related to various chronic health conditions.
species has been reported in obese individuals; however, its role is unknown. We compared the abundance and composition of
species by analyzing feces from 64 healthy control subjects and 88 obese subjects. We isolated one
strain from the feces of a subject with obesity and further analyzed its genetic and molecular features. We found that an increased abundance and higher prevalence of Lactobacillus sakei distinguished the fecal microbiota of the obese group from that of healthy subjects and that it was related to the increased levels of reactive oxygen species (ROS) induced by higher fat intake. The
ob4.1 strain, isolated from the feces of a subject with obesity, showed high catalase activity, which was regulated by oxidative stress at the gene transcription level.
ob4.1 maintained colon epithelial cell adhesion ability under ROS stimulation, and treatment with saturated fatty acid increased colon epithelial ROS levels in a dose-dependent manner; however,
ob4.1 did not change the level of fat-induced colon epithelial ROS. Exposing mice to a high-fat diet revealed that high-fat-diet-induced colon ROS was associated with the increased colonization of
ob4.1 through catalase activity. Four-week supplementation with this strain in mice fed a high-fat diet did not change their body weights or ROS levels. A high-fat diet induces changes in the colon environment by increasing ROS levels, which provides a colonization benefit to an
strain with high catalase activity.
provides many health benefits; its various species are widely used as probiotics. However, an increased abundance of
has been reported in obesity, and the role of
strains in obesity remains unknown. We found a high abundance of the Lactobacillus sakei species in a group of obese subjects and examined its relationship with a high-fat diet and reactive oxygen species (ROS) in the feces. To find the underlying mechanism, we analyzed and characterized an
strain isolated from a severely obese individual. We found that higher gut oxidative stress could link high-fat-diet-induced obesity and
This translational research identifies the roles of the host gut environment in the colonization and survival of
.