A Fully Unsupervised Compartment-on-Demand Platform for Precise Nanoliter Assays of Time-Dependent Steady-State Enzyme Kinetics and Inhibition

The ability to miniaturize biochemical assays in water-in-oil emulsion droplets allows a massive scale-down of reaction volumes, so that high-throughput experimentation can be performed more economically and more efficiently. Generating such droplets in compartment-on-demand (COD) platforms is the b...

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Bibliographic Details

Published in:	Analytical chemistry (Washington) Vol. 85; no. 9; pp. 4761 - 4769
Main Authors:	Gielen, Fabrice, van Vliet, Liisa, Koprowski, Bartosz T, Devenish, Sean R. A, Fischlechner, Martin, Edel, Joshua B, Niu, Xize, deMello, Andrew J, Hollfelder, Florian
Format:	Journal Article
Language:	English
Published:	United States American Chemical Society 07-05-2013
Subjects:	Analytical chemistry beta-Glucosidase - antagonists & inhibitors beta-Glucosidase - metabolism Enzyme kinetics Kinetics Nanotechnology - instrumentation Particle Size Prunus - enzymology Substrates Time Factors
Online Access:	Get full text
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