Application of Statistical Design for the Production of Cellulase by Trichoderma reesei Using Mango Peel

• Published in: Enzyme Research Vol. 2012; no. 2012; pp. 13 - 19
• Main Authors: Saravanan, P., Viruthagiri, T., Muthuvelayudham, R.
• Format: Journal Article
• Language: English
• Published: Cairo, Egypt Hindawi Limiteds 2012; Hindawi Puplishing Corporation; Hindawi Publishing Corporation
• Subjects: Cakes; Cell culture; Cellulase; Enzymes; Flour; Hypocrea jecorina; Mangifera indica; Nutrients; Soybeans; Statistics
• ISSN: 2090-0414; 2090-0406; 2090-0414
• DOI: 10.1155/2012/157643

Optimization of the culture medium for cellulase production using Trichoderma reesei was carried out. The optimization of cellulase production using mango peel as substrate was performed with statistical methodology based on experimental designs. The screening of nine nutrients for their influence on cellulase production is achieved using Plackett-Burman design. Avicel, soybean cake flour, KH2PO4, and CoCl2·6H2O were selected based on their positive influence on cellulase production. The composition of the selected components was optimized using Response Surface Methodology (RSM). The optimum conditions are as follows: Avicel: 25.30 g/L, Soybean cake flour: 23.53 g/L, KH2PO4: 4.90 g/L, and CoCl2·6H2O: 0.95 g/L. These conditions are validated experimentally which revealed an enhanced Cellulase activity of 7.8 IU/mL.