Hydrophilic Interaction Chromatography Using a Meter-Scale Monolithic Silica Capillary Column for Proteomics LC-MS

A meter-scale monolithic silica capillary column modified with urea-functional groups for hydrophilic interaction liquid chromatography (HILIC) was developed for highly efficient separation of biological compounds. We prepared a ureidopropylsilylated monolithic silica capillary column with a minimum...

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Bibliographic Details
Published in:	Analytical chemistry (Washington) Vol. 86; no. 8; pp. 3817 - 3824
Main Authors:	Horie, Kanta, Kamakura, Takeo, Ikegami, Tohru, Wakabayashi, Masaki, Kato, Takashi, Tanaka, Nobuo, Ishihama, Yasushi
Format:	Journal Article
Language:	English
Published:	United States American Chemical Society 15-04-2014
Subjects:	Analytical chemistry Capillarity Chromatography Chromatography, Liquid - instrumentation Chromatography, Liquid - methods HeLa Cells Humans Kinetics Liquid chromatography Mass Spectrometry - methods Neoplasm Proteins - chemistry Nucleosides Peptides Peptides - chemistry Permeability Proteins Proteomics Proteomics - methods Silica Silicon Dioxide Trypsin - chemistry Urea - chemistry
Online Access:	Get full text
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