An Improved Source of Bryostatin 10, Bugula neritina from the Gulf of Aomori, Japan

• Published in: Journal of natural products (Washington, D.C.) Vol. 58; no. 12; pp. 1868 - 1875
• Main Authors: Kamano, Yoshiaki, Zhang, Hui-Ping, Hino, Akiya, Yoshida, Motoyuki, Pettit, George R, Herald, Cherry L, Itokawa, Hideji
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 01-12-1995; Glendale, AZ American Society of Pharmacognosy
• Subjects: AGENTES ANTINEOPLASTICOS; ANALISIS CUANTITATIVO; ANALYSE QUANTITATIVE; ANIMAL AQUATIQUE; ANIMALES ACUATICOS; Animals; ANTINEOPLASTIC AGENTS; AQUATIC ANIMALS; Biological and medical sciences; Bryostatins; Bryozoa - chemistry; Cattle; Cells, Cultured; CHEMICAL COMPOSITION; COMPOSICION QUIMICA; COMPOSITION CHIMIQUE; ESPECTROMETRIA; General pharmacology; GULF OF OHZUCHI; GULF OF SAGAMI; INVERTEBRADOS; INVERTEBRATES; INVERTEBRE; Japan; LACTONAS; LACTONE; LACTONES; Lactones - chemistry; Lactones - isolation & purification; Macrolides; Magnetic Resonance Spectroscopy; MAR DEL JAPON; Medical sciences; MEDICAMENT CYTOSTATIQUE; MER DU JAPON; MOLECULAR CONFORMATION; MOLECULAR STRUCTURE; Pharmacognosy. Homeopathy. Health food; Pharmacology. Drug treatments; QUANTITATIVE ANALYSIS; SEA OF JAPAN; SPECTRAL ANALYSIS; SPECTROMETRIE; SPECTROMETRY; Steroids - biosynthesis; Asia; Japan; Antineoplastic agent; Bryozoa; Animal origin; Lactone; Tetracyclic compound; Oxygen heterocycle; Macrolide; Lophophoria; Macrocycle; Marine environment; Isolation; Invertebrata; Structural analysis
• ISSN: 0163-3864; 1520-6025
• DOI: 10.1021/np50126a009

Bryostatins have been isolated from diverse Japanese coastal specimens of Bugula neritina guided by inhibitory activity against fertilized sea urchin egg cell division. B. neritina from the Gulf of Aomori, Japan, has been found to contain bryostatin 10 [1b] in high yield (10(-3)%) for this class of compounds. The 1H- and 13C-nmr signals of bryostatin 10 [1b] were reassigned by 2D nmr techniques. The conformation of bryostatin 10 [1b] in solution was revealed by nmr studies. This compound also exhibited activity in a steroidogenesis assay by increasing the production of adrenocortical hormones nearly twofold.