Peptide Labeling with Isobaric Tags Yields Higher Identification Rates Using iTRAQ 4-Plex Compared to TMT 6-Plex and iTRAQ 8-Plex on LTQ Orbitrap

Peptide labeling with isobaric tags has become a popular technique in quantitative shotgun proteomics. Using two different samples viz. a protein mixture and HeLa extracts, we show that three commercially available isobaric tags differ with regard to peptide identification rates: The number of ident...

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Bibliographic Details
Published in:	Analytical chemistry (Washington) Vol. 82; no. 15; pp. 6549 - 6558
Main Authors:	Pichler, Peter, Köcher, Thomas, Holzmann, Johann, Mazanek, Michael, Taus, Thomas, Ammerer, Gustav, Mechtler, Karl
Format:	Journal Article
Language:	English
Published:	Washington, DC American Chemical Society 01-08-2010
Subjects:	Analytical chemistry Biological and medical sciences Comparative analysis Diverse techniques Fundamental and applied biological sciences. Psychology HeLa Cells Humans Ions Molecular and cellular biology Peptides Peptides - chemistry Piperazines - chemistry Piperidines - chemistry Proteins Proteomics Proteomics - methods Succinimides - chemistry Collisional activation Peptides Sample Standard Identification Method Precursor Mixture Protein Sample preparation Proteomics Yield Technique Quantitative analysis
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