Dual Expression System Suitable for High-Throughput Fluorescence-Based Screening and Production of Soluble Proteins

Many studies that aim to characterize the proteome structurally or functionally require the production of pure protein in a high-throughput format. We have developed a fast and flexible integrated system for cloning, protein expression in Escherichia coli, solubility screening and purification that...

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Bibliographic Details
Published in:	Journal of proteome research Vol. 4; no. 6; pp. 2137 - 2147
Main Authors:	Braud, Sandrine, Moutiez, Mireille, Belin, Pascal, Abello, Nicolas, Drevet, Pascal, Zinn-Justin, Sophie, Courçon, Marie, Masson, Cédric, Dassa, Janie, Charbonnier, Jean-Baptiste, Boulain, Jean-Claude, Ménez, André, Genet, Roger, Gondry, Muriel
Format:	Journal Article
Language:	English
Published:	United States American Chemical Society 01-11-2005
Subjects:	Biochemistry Biochemistry, Molecular Biology Biotechnology Blotting, Western Cloning, Molecular Codon, Terminator DNA - chemistry Electrophoresis, Polyacrylamide Gel Escherichia coli - genetics Escherichia coli - metabolism Escherichia coli Proteins - chemistry Gene Expression Regulation Gene Expression Regulation, Bacterial Genes, Suppressor Genetic Vectors Glutathione Transferase - metabolism Life Sciences Proteins - chemistry Proteome Proteomics - methods Recombinant Fusion Proteins - metabolism Recombination, Genetic RNA, Transfer - metabolism Solubility Spectrometry, Fluorescence Temperature S·tag fusion proteins fluorescence high-throughput screening recombination cloning protein expression amber suppressor
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