Human granulocyte activity against moxalactam-induced filamentous forms of Pseudomonas aeruginosa

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Published in:	Antimicrobial Agents and Chemotherapy Vol. 32; no. 10; pp. 1565 - 1570
Main Authors:	HAMMER, M. C, BALTCH, A. L, SMITH, R. P, CONROY, J. V, BISHOP, M, MICHELSEN, P, HILL, L
Format:	Journal Article
Language:	English
Published:	Washington, DC American Society for Microbiology 01-10-1988
Subjects:	Biological and medical sciences Blood Bactericidal Activity Blood Bactericidal Activity - drug effects Cytoskeleton - drug effects Fundamental and applied biological sciences. Psychology Fundamental immunology Humans Immunobiology In Vitro Techniques Microscopy, Electron Moxalactam Moxalactam - pharmacology Myeloid cells: ontogeny, maturation, markers, receptors Neutrophils Neutrophils - drug effects Neutrophils - physiology Phagocytosis - drug effects Pseudomonas aeruginosa Pseudomonas aeruginosa - drug effects Pseudomonas aeruginosa - ultrastructure Research Article Ruthenium Red Pseudomonadales Human Rod Granulocyte Lactam Host agent relation Bactericidal effect Antibiotic Bacteria Pseudomonadaceae Pseudomonas aeruginosa Kinetics Phagocytosis
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Abstract	Classifications Services AAC Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue AAC About AAC Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy AAC RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • journals@asmusa.org Print ISSN: 0066-4804 Online ISSN: 1098-6596 Copyright © 2014 by the American Society for Microbiology. For an alternate route to AAC .asm.org, visit: AAC
AbstractList	The purpose of this investigation was to study the kinetics of human granulocyte (polymorphonuclear leukocyte) phagocytosis and bactericidal activity against beta-lactam antibiotic (moxalactam)-induced filamentous bacterial forms of Pseudomonas aeruginosa. Ultrastructural observations of rod and filamentous forms of P. aeruginosa and their interaction with polymorphonuclear leukocytes are presented. Growth of P. aeruginosa 1348A in the presence of 4 micrograms of moxalactam per ml (one-fourth the MIC) resulted in filamentous forms. Phagocytosis of 75Se-radiolabeled filaments was more efficient than that of rods during the first 20 min of the assay; subsequently, phagocytosis of both forms was equal. The polymorphonuclear leukocyte bactericidal activities against both forms, which were standardized to equal bacterial particle and viable-cell counts, were equivalent. Considering the greater size and mass of filaments compared with those of rods, we concluded that filaments are more susceptible to both phagocytosis and killing than are bacillary forms. Classifications Services AAC Citing Articles Google Scholar PubMed Related Content Social Bookmarking CiteULike Delicious Digg Facebook Google+ Mendeley Reddit StumbleUpon Twitter current issue AAC About AAC Subscribers Authors Reviewers Advertisers Inquiries from the Press Permissions & Commercial Reprints ASM Journals Public Access Policy AAC RSS Feeds 1752 N Street N.W. • Washington DC 20036 202.737.3600 • 202.942.9355 fax • journals@asmusa.org Print ISSN: 0066-4804 Online ISSN: 1098-6596 Copyright © 2014 by the American Society for Microbiology. For an alternate route to AAC .asm.org, visit: AAC The purpose of this investigation was to study the kinetics of human granulocyte (polymorphonuclear leukocyte) phagocytosis and bactericidal activity against beta-lactam antibiotic (moxalactam)-induced filamentous bacterial forms of Pseudomonas aeruginosa . Ultrastructural observations of rod and filamentous forms of P. aeruginosa and their interaction with polymorphonuclear leukocytes are presented. Growth of P. aeruginosa 1348A in the presence of 4 mu g of moxalactam per ml (one-fourth the MIC) resulted in filamentous forms. Phagocytosis of super(75)Se-radiolabeled filaments was more efficient than that of rods during the first 20 min of the assay; subsequently, phagocytosis of both forms was equal. The polymorphonuclear leukocyte bactericidal activities against both forms, which were standardized to equal bacterial particle and viable-cell counts, were equivalent. Considering the greater size and mass of filaments compared with those of rods, we concluded that filaments are more susceptible to both phagocytosis and killing than are bacillary forms.
Author	L Hill A L Baltch J V Conroy M C Hammer R P Smith P Michelsen M Bishop
AuthorAffiliation	Department of Medicine, Veterans Administration Medical Center, Albany, New York
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SubjectTerms	Biological and medical sciences Blood Bactericidal Activity Blood Bactericidal Activity - drug effects Cytoskeleton - drug effects Fundamental and applied biological sciences. Psychology Fundamental immunology Humans Immunobiology In Vitro Techniques Microscopy, Electron Moxalactam Moxalactam - pharmacology Myeloid cells: ontogeny, maturation, markers, receptors Neutrophils Neutrophils - drug effects Neutrophils - physiology Phagocytosis - drug effects Pseudomonas aeruginosa Pseudomonas aeruginosa - drug effects Pseudomonas aeruginosa - ultrastructure Research Article Ruthenium Red
Title	Human granulocyte activity against moxalactam-induced filamentous forms of Pseudomonas aeruginosa
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