Calcium Uncaging with Visible Light

We have designed a nitroaromatic photochemical protecting group that absorbs visible light in the violet-blue range. The chromophore is a dinitro derivative of bisstyrylthiophene (or BIST) that absorbs light very effectively (ε440 = 66,000 M–1 cm–1 and two-photon cross section of 350 GM at 775 nm)....

Full description

Saved in:

Bibliographic Details
Published in:	Journal of the American Chemical Society Vol. 138; no. 11; pp. 3687 - 3693
Main Authors:	Agarwal, Hitesh K, Janicek, Radoslav, Chi, San-Hui, Perry, Joseph W, Niggli, Ernst, Ellis-Davies, Graham C. R
Format:	Journal Article
Language:	English
Published:	United States American Chemical Society 23-03-2016
Subjects:	Calcium Compounds - chemical synthesis Calcium Compounds - chemistry Chelating Agents - chemistry Egtazic Acid - chemistry Light Photochemical Processes Thiophenes - chemical synthesis Thiophenes - chemistry
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Description
Summary:	We have designed a nitroaromatic photochemical protecting group that absorbs visible light in the violet-blue range. The chromophore is a dinitro derivative of bisstyrylthiophene (or BIST) that absorbs light very effectively (ε440 = 66,000 M–1 cm–1 and two-photon cross section of 350 GM at 775 nm). We developed a “caged calcium” molecule by conjugation of BIST to a Ca2+ chelator that upon laser flash photolysis rapidly releases Ca2+ in <0.2 ms. Using the patch-clamp method the optical probe, loaded with Ca2+, was delivered into acutely isolated mouse cardiac myocytes, where either one- and two-photon uncaging of Ca2+ induced highly local or cell-wide physiological Ca2+ signaling events.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 These authors contributed equally to this work.
ISSN:	0002-7863 1520-5126
DOI:	10.1021/jacs.5b11606