A Homogeneous and Noncompetitive Immunoassay Based on the Enhanced Fluorescence Resonance Energy Transfer by Leucine Zipper Interaction

Fluorescence resonance energy transfer (FRET) between two GFP variants is a powerful technique to describe protein−protein interaction in a biological system. However, it has a limitation that the two variants tethered to the respective proteins have to be in sufficient proximity upon binding, which...

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Bibliographic Details
Published in:	Analytical chemistry (Washington) Vol. 74; no. 22; pp. 5786 - 5792
Main Authors:	Ohiro, Yoshiyuki, Arai, Ryoichi, Ueda, Hiroshi, Nagamune, Teruyuki
Format:	Journal Article
Language:	English
Published:	Washington, DC American Chemical Society 15-11-2002
Subjects:	Analytical chemistry Chemistry Energy Transfer Enzyme-Linked Immunosorbent Assay Exact sciences and technology Fluorescence Green Fluorescent Proteins Humans Immunoassay - methods Leucine Zippers Luminescent Proteins - chemistry Miscellaneous Plasmids - genetics Proteins Recombinant Fusion Proteins - chemistry Recombinant Proteins - chemistry Spectrometric and optical methods Spectrometry, Fluorescence Investigation method Zinc finger structure Green fluorescent protein Sandwich structure Fluorescence Resonance energy Leucine Energy transfer Fluorescence spectrometry Immunological method
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