Rapid Preparation of Procyanidins B2 and C1 from Granny Smith Apples by Using Low Pressure Column Chromatography and Identification of Their Oligomeric Procyanidins

• Published in: Journal of agricultural and food chemistry Vol. 56; no. 6; pp. 2096 - 2101
• Main Authors: Xiao, Jun-Song, Liu, Liang, Wu, Hua, Xie, Bi-Jun, Yang, Er-Ning, Sun, Zhi-Da
• Format: Journal Article
• Language: English
• Published: Washington, DC American Chemical Society 26-03-2008
• Subjects: apples; Biflavonoids - isolation & purification; Biological and medical sciences; Catechin - isolation & purification; Chemical Composition of Foods/Feeds; chromatography; Chromatography - methods; Chromatography, Liquid; epicatechin; extraction; Food industries; fractionation; Fruit - chemistry; Fruit and vegetable industries; fruit composition; Fundamental and applied biological sciences. Psychology; Granny Smith apple; Malus - chemistry; new methods; polymers; preparation; Proanthocyanidins - analysis; Proanthocyanidins - chemistry; Proanthocyanidins - isolation & purification; procyanidin; procyanidins; rapid methods; reference standards; Reproducibility of Results; Spectrometry, Mass, Electrospray Ionization; Toyopearl TSK HW-40s column; Granny Smith apple; procyanidin; Toyopearl TSK HW-40s column; preparation; Polyphenol; Apple; Fruit; Preparation; Low pressure; Identification; Proanthocyanidin; Column chromatography; Flavonoid
• ISSN: 0021-8561; 1520-5118
• DOI: 10.1021/jf072679x

Research in the field of procyanidins is always hindered by the lack of procyanidin standards, and the preparation of procyanidins, especially in large scale, remains difficult and time-consuming. Commercial sources of procyanidin standards are scarce. In this study, a rapid preparation method of procyanidins by using low-pressure column chromatography was developed. Procyanidins in Granny Smith apples were extracted with boiled water and purified on an ADS-17 macroporous resin column to obtain a Granny Smith apple procyanidin extract (GSE). GSE was fractionated according to its degree of polymerization on a Toyopearl TSK HW-40s column. Procyanidins B2 (epicatechin-(4β−8)-epicatechin) and C1 (epicatechin-(4β−8)-epicatechin-(4β−8)-epicatechin) were prepared without HPLC separation. Oligomeric procyanidins from Granny Smith apples were also identified by liquid chromatography−electrospray ionization−mass spectrometry.