Label-Free Quantitative Proteomic Analysis of Systemic Responses to Local Wounding and Virus Infection in Arabidopsis thaliana

• Published in: Journal of proteome research Vol. 12; no. 6; pp. 2491 - 2503
• Main Authors: Niehl, Annette, Zhang, Zhe Jenny, Kuiper, Martin, Peck, Scott C, Heinlein, Manfred
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 07-06-2013
• Subjects: Arabidopsis - genetics; Arabidopsis - immunology; Arabidopsis - virology; Arabidopsis Proteins - genetics; Arabidopsis Proteins - immunology; Arabidopsis Proteins - isolation & purification; Biochemistry, Molecular Biology; Cellular Biology; Chromatography, Liquid; Gene Expression Profiling; Gene Expression Regulation, Plant; Life Sciences; Molecular biology; Molecular Sequence Annotation; Plant Diseases - genetics; Plant Diseases - immunology; Plant Diseases - virology; Plant Leaves - genetics; Plant Leaves - immunology; Plant Leaves - virology; Proteomics; Signal Transduction; Tandem Mass Spectrometry; Tobamovirus - immunology; wound; Arabidopsis; virus; systemic signaling; label-free quantification
• DOI: 10.1021/pr3010698
• EISSN: 1535-3907
• ISSN: 1535-3893

Plants are continuously exposed to changing environmental conditions and must, as sessile organisms, possess sophisticated acclimative mechanisms. To gain insight into systemic responses to local virus infection or wounding, we performed comparative LC–MS/MS protein profiling of distal, virus-free leaves four and five days after local inoculation of Arabidopsis thaliana plants with either Oilseed rape mosaic virus (ORMV) or inoculation buffer alone. Our study revealed biomarkers for systemic signaling in response to wounding and compatible virus infection in Arabidopsis, which should prove useful in further addressing the trigger-specific systemic response network and the elusive systemic signals. We observed responses common to ORMV and mock treatment as well as protein profile changes that are specific to local virus infection or mechanical wounding (mock treatment) alone, which provides evidence for the existence of more than one systemic signal to induce these distinct changes. Comparison of the systemic responses between time points indicated that the responses build up over time. Our data indicate stress-specific changes in proteins involved in jasmonic and abscisic acid signaling, intracellular transport, compartmentalization of enzyme activities, protein folding and synthesis, and energy and carbohydrate metabolism. In addition, a virus-triggered systemic signal appears to suppress antiviral host defense.