Qualitative and Quantitative Evaluation of Protein Extraction Protocols for Apple and Strawberry Fruit Suitable for Two-Dimensional Electrophoresis and Mass Spectrometry Analysis

A modified phenol-based protocol and a phenol-free protocol that involves hot SDS extraction followed by TCA precipitation in acetone were qualitatively and quantitatively compared and evaluated on apple peel and strawberry fruit. The phenol protocol resulted in significantly higher protein yields o...

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Bibliographic Details
Published in:	Journal of agricultural and food chemistry Vol. 55; no. 5; pp. 1663 - 1673
Main Authors:	Zheng, Qifa, Song, Jun, Doncaster, Kristen, Rowland, Elden, Byers, David M
Format:	Journal Article
Language:	English
Published:	Washington, DC American Chemical Society 07-03-2007
Subjects:	Amino Acid Sequence Biological and medical sciences Electrophoresis, Gel, Two-Dimensional - methods Food engineering Food industries Fragaria - chemistry Fruit - chemistry Fruit and vegetable industries Fundamental and applied biological sciences. Psychology General aspects Malus - chemistry Mass Spectrometry - methods Molecular Sequence Data Phenol Plant Proteins - chemistry Plant Proteins - isolation & purification Sodium Dodecyl Sulfate Evaluation Fragaria Fruit Rosaceae Protein Lauryl sulfate fruit tissue Tissue LC-MS/MS Phenol Apple two-dimensional electrophoresis Dicotyledones Strawberry Sodium sulfate Angiospermae Phenols Protein extraction sodium dodecyl sulfate Spermatophyta Extraction Two dimensional electrophoresis Mass spectrometry
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