NMR studies of differences in the conformations and dynamics of ligand complexes formed with mutant dihydrofolate reductases

Two mutants of Lactobacillus casei dihydrofolate reductase, Trp 21---Leu and Asp 26---Glu, have been prepared by using site-directed mutagenesis methods, and their ligand binding and structural properties have been compared with those of the wild-type enzyme. 1H, 13C, and 31P NMR studies have been c...

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Bibliographic Details
Published in:	Biochemistry (Easton) Vol. 28; no. 3; pp. 1353 - 1362
Main Authors:	Birdsall, B, Andrews, J, Ostler, G, Tendler, S. J. B, Feeney, J, Roberts, G. C. K, Davies, R. W, Cheung, H. T. A
Format:	Journal Article
Language:	English
Published:	United States American Chemical Society 07-02-1989
Subjects:	550601 - Medicine- Unsealed Radionuclides in Diagnostics AMINO ACIDS ANTIMETABOLITES ANTINEOPLASTIC DRUGS AROMATICS ASPARTIC ACID AZAARENES AZOLES BACTERIA BARYONS CARBON 13 CARBON ISOTOPES CARBOXYLIC ACIDS CHEMICAL REACTIONS CHEMICAL SHIFT Cloning, Molecular COENZYMES CROSS-LINKING DRUGS ELEMENTARY PARTICLES ENZYME INHIBITORS ENZYMES Escherichia coli - genetics EVEN-ODD NUCLEI FERMIONS Genes Genes, Bacterial GLUTAMIC ACID HADRONS HETEROCYCLIC ACIDS HETEROCYCLIC COMPOUNDS INDOLES ISOTOPES LACTOBACILLUS Lactobacillus casei - enzymology Lactobacillus casei - genetics LEUCINE LIGANDS LIGHT NUCLEI MAGNETIC RESONANCE Magnetic Resonance Spectroscopy METHOTREXATE Methotrexate - metabolism MICROORGANISMS MUTAGENESIS MUTANTS Mutation NADP NUCLEAR MAGNETIC RESONANCE NUCLEI NUCLEONS NUCLEOTIDES ODD-EVEN NUCLEI ORGANIC ACIDS ORGANIC COMPOUNDS ORGANIC NITROGEN COMPOUNDS OXIDOREDUCTASES PHOSPHORUS 31 PHOSPHORUS ISOTOPES POLYMERIZATION Protein Binding Protein Conformation PROTONS PYRROLES RADIOLOGY AND NUCLEAR MEDICINE RESONANCE STABLE ISOTOPES SUBSTRATES Tetrahydrofolate Dehydrogenase - genetics Tetrahydrofolate Dehydrogenase - metabolism TRYPTOPHAN
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