Intrinsic Dynamics in ECFP and Cerulean Control Fluorescence Quantum Yield

Enhanced cyan fluorescent protein (ECFP) and its variant Cerulean are genetically encoded fluorophores widely used as donors in FRET-based cell imaging experiments. First, we have confirmed through denaturation experiments that the double-peak spectroscopic signature of these fluorescent proteins or...

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Bibliographic Details

Published in:	Biochemistry (Easton) Vol. 48; no. 42; pp. 10038 - 10046
Main Authors:	Lelimousin, Mickaël, Noirclerc-Savoye, Marjolaine, Lazareno-Saez, Christelle, Paetzold, Bernhard, Le Vot, Sophie, Chazal, Richard, Macheboeuf, Pauline, Field, Martin J, Bourgeois, Dominique, Royant, Antoine
Format:	Journal Article
Language:	English
Published:	United States American Chemical Society 27-10-2009
Subjects:	Computer Simulation Crystallography, X-Ray Fluorescence Resonance Energy Transfer Fluorescent Dyes - chemistry Green Fluorescent Proteins - chemistry Green Fluorescent Proteins - metabolism Hydrogen-Ion Concentration Models, Molecular Protein Conformation Recombinant Proteins - chemistry Recombinant Proteins - metabolism Tryptophan - chemistry
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