Halothane Binding Proteome in Human Brain Cortex

• Published in: Journal of proteome research Vol. 6; no. 2; pp. 582 - 592
• Main Authors: Pan, Jonathan Z, Xi, Jin, Tobias, John W, Eckenhoff, Maryellen F, Eckenhoff, Roderic G
• Format: Journal Article
• Language: English
• Published: United States American Chemical Society 01-02-2007
• Subjects: Cerebral Cortex - chemistry; Cerebral Cortex - metabolism; Electrophoresis, Gel, Two-Dimensional; Enzymes - chemistry; Enzymes - isolation & purification; Enzymes - metabolism; Halothane - metabolism; Humans; Nerve Tissue Proteins - chemistry; Nerve Tissue Proteins - classification; Nerve Tissue Proteins - isolation & purification; Nerve Tissue Proteins - metabolism; Protein Binding; Proteome
• ISSN: 1535-3893; 1535-3907
• DOI: 10.1021/pr060311u

Inhaled anesthetics bind specifically to a wide variety of proteins in the brain. This set of proteins must include those that contribute to the physiological and behavioral phenotypes of anesthesia and the related side effects. To identify the anesthetic-binding targets and functional pathways associated with these targets in human brain, halothane photolabeling and two-dimensional (2D) gel electrophoresis were used. Both membrane and soluble proteins from human temporal cortex were prepared. More than 300 membrane and 400 soluble protein spots were detected on the stained blots, of which 23 membrane and 34 soluble proteins were labeled by halothane and identified by mass spectroscopy. Their functional classification reveals five groups, including carbohydrate metabolism, protein folding, oxidative phosphorylation, nucleoside triphosphatase, and dimer/kinase activity with different correlative stringency. When network analysis of the interaction between these protein molecules is used, the weighted interaction accentuates the cellular protein components important in cell growth and proliferation, cell cycle and cell death, and cell−cell signaling and interactions, although no pathway was specific. This study provides evidence for multiple anesthetic binding targets and suggests potential pathways involved in their actions. Keywords: anesthetic • protein binding • protein expression • 2D gel electrophoresis • CNS • soluble proteins • membrane proteins • functional classification • network analysis