Peroxynitrite Inactivation of Human Cytochrome P450s 2B6 and 2E1: Heme Modification and Site-Specific Nitrotyrosine Formation

This study examined the reaction of peroxynitrite (PN) with two human cytochrome P450s, P450 2B6 (2B6) and P450 2E1 (2E1). After the reaction with PN, the NADPH/reductase-supported 7-ethoxy-4-(trifluoromethyl)coumarin (EFC) deethylation activity of both P450s was decreased in a concentration-depende...

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Bibliographic Details
Published in:	Chemical research in toxicology Vol. 20; no. 11; pp. 1612 - 1622
Main Authors:	Lin, Hsia-lien, Myshkin, Eugene, Waskell, Lucy, Hollenberg, Paul F
Format:	Journal Article
Language:	English
Published:	United States American Chemical Society 01-11-2007
Subjects:	Amino Acid Sequence Aryl Hydrocarbon Hydroxylases - antagonists & inhibitors Aryl Hydrocarbon Hydroxylases - chemistry Aryl Hydrocarbon Hydroxylases - metabolism Carbon Monoxide - chemistry Cytochrome P-450 CYP2B6 Cytochrome P-450 CYP2E1 - chemistry Cytochrome P-450 CYP2E1 - metabolism Cytochrome P-450 CYP2E1 Inhibitors Enzyme Inhibitors - pharmacology Heme - chemistry Humans Models, Molecular Molecular Sequence Data Oxidoreductases, N-Demethylating - antagonists & inhibitors Oxidoreductases, N-Demethylating - chemistry Oxidoreductases, N-Demethylating - metabolism Peroxynitrous Acid - pharmacology tert-Butylhydroperoxide - pharmacology Tyrosine - analogs & derivatives Tyrosine - biosynthesis
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